Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 613-739-4 | CAS number: 65039-09-0
Endpoint summary
Administrative data
Description of key information
Key value for chemical safety assessment
Skin sensitisation
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed (not sensitising)
- Additional information:
There are studies to determine the sensitizing potential of 1-ethyl-3-methylimidazolium chlorid available. In the combined local lymph node assay (LLNA) and irritancy assay (IRR) female BALB/c mice were applied dermally with concentrations of 6.25, 12.5, 25 and 50 % in olive oil (4:1; AOO). In the LLNA, EMI at concentrations of 6.25%-50% did not induce significant increase in lymph node cell proliferation when compared to the vehicle control. EMI was not an irritant at concentrations up to 50%, as demonstrated by a lack of effect on ear thickness at 24 hr following the last dermal exposure.
To confirm the negative LLNA response, the Mouse Ear Swelling Test (MEST) was performed following exposure to EMI. In the sensitization phase two concentrations of EMI (255 and 50%) were used, and a concentration of 50% was applied during the challenge phase. There was no significant difference in the percent ear swelling at 24 and 48 hr following challenge in mice treated with EMI when comparred to the vehicle irritancy control (VHIC).
In conclusion, under the experimental conditions utilized in this study of female BALB/c mice, EMI was negative in the LLNA, the irritancy assay, and the MEST (NTP, 2010).
Migrated from Short description of key information:
LLNA: not sensitising (NTP, 2010)
MEST: not sensitising (NTP, 2010)
Justification for classification or non-classification
Based on the results, the test item is no subject to classification and labelling according to Regulation (EC) No 1272/2008 (CLP).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
