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EC number: 940-742-0 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- migrated information: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Study period:
- from Sempember 24, 2007 to November 29, 2007
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: This study was carried out in accordance with internationally valid GLP principles.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 007
- Report date:
- 2007
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Humic acids, potassium salts
- EC Number:
- 271-030-1
- EC Name:
- Humic acids, potassium salts
- Cas Number:
- 68514-28-3
- IUPAC Name:
- 68514-28-3
- Details on test material:
- - Name of test material (as cited in study report): Humic acids, potassium salts
- Molecular formula: not known - UVCB substance
- Molecular weight: not known - UVCB substance
- Substance type: technical product
- Physical state: solid
- Lot/batch No.: 16. 5. 2007/R
- Expiration date of the lot/batch: 05/2022
- Stability under test conditions: stable
- Storage condition of test material: dry conditions
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 61 - 150 mg/L (nominal concentration)
- Sampling method:
The analytical determination of the test substance concentrations was performed in each test concentrations at the beginning and at the end of the test.
- Sample storage conditions before analysis:
The samples were analysed on the same day as sampling.
All samples were stored at laboratory temperature.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- The test substance was soluble in the dilution water in concentration needed for the test. The stock solution of the test substance was prepared in the dilution water. 200 mg of the test substance was weighed into 2000 mL of the dilution water for preliminary test and 2000 mg of the test substance was weighed into 2000 mL of the dilution water for limit test. The concentrations of solutions used in the preliminary and the limit test were obtained by dilution of the stock solution with dilution water. The exact concentrations were analyzed.
Test organisms
- Test organisms (species):
- Poecilia reticulata
- Details on test organisms:
- TEST ORGANISM
- Common name: Poecilia reticulata
- Strain:
- Source: certified supplier BioTest s.r.o., Konárovice (Czech Republic).
- Age at study initiation (mean and range, SD): 3-4months old
- Length at study initiation (length definition, mean, range and SD): 2.0-3.0 cm
- Weight at study initiation (mean and range, SD): preliminary test 7 fishes cca 1g, limit test: 10 fishes cca 0.7 g
- Method of breeding: The fish were kept in all-glass aquaria
- Feeding during test: no
- Food type:
- Amount:
- Frequency:
ACCLIMATION
- Acclimation period: Fish were acclimatized for 7 days
- Acclimation conditions (same as test or not): in dilution water
- Type and amount of food: Diet: “Tropica” - feed for aquarium fish, ad libitum and sometimes live daphnia. The fishes were not fed during 72 hours before the test.
- Feeding frequency: ad libitum
- Health during acclimation (any mortality observed): healthy animals
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Post exposure observation period:
- none
Test conditions
- Hardness:
- Content of (Ca + Mg) ions: 2.63 mmol/L (preliminary test)
Content of (CA + Mg) ions: 2.59 mmol/L (main test) - Test temperature:
- 22±2°C, with a variation of ± 1°C at each individual test
- pH:
- 7.8 - 7.9 (at the beginning)
- Dissolved oxygen:
- 8.5 mg/L at temperature 21.2 °C (main test)
- Nominal and measured concentrations:
- see table 1
- Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Type: open
- Material, size, headspace, fill volume: Glass vessels, size 3L, volume 2L
- Aeration: without
- No. of organisms per vessel: 7 for the preliminary test, 10 for the limit test
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- No. of vessels per vehicle control (replicates): none
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water:
Standard dilution water was prepared just before the test by admixing 2.5 mL of stored solutions A, B, C and D in 1000 mL of distilled water.
A. 117.6 g CaCl2.2H20 in 1L distilled water
B. 49.3 g MgSO4.7H2O in 1L distilled water
C. 25.9 g NaHCO3 in 1L distilled water
D. 2.3 g KCl in 1L distilled water
The dilution water should be of pH 7.8 ± 0.2. It is saturated by oxygen by the aeration at temperature of 20 - 24°C. The content of sum Ca + Mg ions should have the value of 2.5 mmol.L-1.
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: daily
- Light intensity: daylight - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 24 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 128 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 128 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 72 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 128 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 128 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 24 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 128 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 128 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 128 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 128 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 24 h
- Dose descriptor:
- LC100
- Effect conc.:
- > 128 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 48 h
- Dose descriptor:
- LC100
- Effect conc.:
- > 128 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 72 h
- Dose descriptor:
- LC100
- Effect conc.:
- > 128 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC100
- Effect conc.:
- > 128 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- - Behavioural abnormalities: no
- Mortality of control: no
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values:
The analytical results showed, that the composition of the dilution water has influence on the solubility of the test substance and the part of the test substance was precipitated at preparation of the stock solution. For this reason concentrations measured at the beginning of the test are lower than nominal concentrations. - Results with reference substance (positive control):
- Reference test
The sensitivity of the test species and correctness of test performance is periodically verified in three-month period by testing the reference substance, potassium dichromate.
The results of verification test with K2Cr2O7, carried out in period from 17.9. to 21.9.2007, are the following:
48h – LC50 = 141 mg.L-1 (95 % confidence limit: 133 – 148 mg.L-1)
96h – LC50 = 133 mg.L-1 (95 % confidence limit: 131 – 135 mg.L-1)
The results of the last interlaboratory test arranged by Centre for Assessment of Laboratories of T.G.Masaryk Water Research Institute Prague in 2006 for potassium dichromate were:
96 h - LC50 = 96 – 154 mg.L-1
The mortality of test organisms in the reference substance solution meets criteria determined by the interlaboratory test.
Any other information on results incl. tables
- Sublethal observations / clinical signs:
Validity of the test
Test quality criteria
These quality criteria are given in the guideline.
The result of test should satisfy quality criteria as follows:
- Mortality in the control must not exceed 10 % (or one fish if less than ten are used) by the end of the test.
- The dissolved oxygen concentration must be at least 60 % of initial value.
- The measured values of pH solution should not change more than 1 pH unit.
- The test substance concentration in test mixtures should not decrease under 80 % of the initial concentration.
Quality criteria at points 1, 2, 3 and 4 were fulfilled.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Executive summary:
The test substance was tested for acute toxicity on fish Poecilia reticulata.
The test was performed according to EU Method C.1., Acute toxicity for fish, Directive 92/69/EEC. Published in O.J. L 383A, 1992.
The water solubility of the test substance is 279 mg·L-1 (see Study No. 26/07/46: Humic acids, potassium salts - Water solubility).
The preliminary test was performed in range of test substance nominal concentrations 1 – 100 mg·L-1. The analytical results showed, that the composition of the dilution water has influence on the solubility of the test substance and the part of the test substance is precipitated at preparation of the stock solution. For this reason concentrations measured at the beginning of the test are lower than nominal concentrations. Based on no toxicity of the test substance found in preliminary test, the limit test was performed.
The limit test was performed in range of test substance nominal concentrations 61 – 150 mg·L-1. The test was performed as static.
With regards to analytical results obtained by preliminary test, the analytical determination of the test substance concentrations was performed in each test concentrations at the beginning and at the end of the test.
For all evaluations and results were used the concentrations measured at the beginning of the test.
Test results:
24h – LC50 > 128 mg·L-1 (determined concentration)
48h – LC50 > 128 mg·L-1 (determined concentration)
72h – LC50 > 128 mg·L-1 (determined concentration)
96h – LC50 > 128 mg·L-1 (determined concentration)
24h – NOEC = 128 mg·L-1 (determined concentration)
48h – NOEC = 128 mg·L-1 (determined concentration)
72h – NOEC = 128 mg·L-1 (determined concentration)
96h – NOEC = 128 mg·L-1 (determined concentration)
24h – LC100 > 128 mg·L-1 (determined concentration)
48h – LC100 > 128 mg·L-1 (determined concentration)
72h – LC100 > 128 mg·L-1 (determined concentration)
96h – LC100 > 128 mg·L-1 (determined concentration)
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