4-[(4-chloro-6-{[(3 or 4)-sulfophenyl]amino}-1,3,5-triazin-2-yl)amino]-2-{[1-ethyl-2-hydroxy-4-methyl-6-oxo-5-(sulfonatomethyl)-1,6-dihydropyridin-3-yl]diazenyl}benzenesulfonic acid, lithium sodium salts

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

48 hours

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes

Test organisms (species):

Daphnia magna

Details on test organisms:

- Source: Aquatic BioSystems (Fort Collins, CO)

Test type:

static

Water media type:

freshwater

Total exposure duration:

48 h

Post exposure observation period:

Day 0, Day 1 and Day 2

Hardness:

104 mg/L as CaCO3

Test temperature:

19.2-21.1˚C

pH:

7.6-8.2

Dissolved oxygen:

9.0-9.5 mg/L

Nominal and measured concentrations:

Nominal concentrations of the test item are 6.25 mg/L, 12.5 mg/L, 25mg/L, 50 mg/L and 100mg/L

Details on test conditions:

- Test vessel: ~250 mL glass beakers
- Test volume: 200 mL
- Aeration: None
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Photoperiod: 16-hr light: 8-hr dark with a 30 minute transition period
- Light intensity: 687-773 lux

Reference substance (positive control):

yes

Remarks:

Zinc sulphate heptahydrate (CAS NO. 7446-20-0)

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

ca. 70.7 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Results with reference substance (positive control):

- LC50 for 48hr: 0.48 mg Zn2+/L

The 0% of control organisms were immobilised and the dissolved oxygen concentration at the end of the test was 9.0-9.1 mgO2/L. The initial pH measurements did not differ from the final measurements by more than 1.5 units in each test. The color of the test solutions remained the same throughout the test..

Table 1. Number and Percentage of Daphnia Immobilised after 24-hr Exposure

Nominal Concentration of Test Item (mg/L)	Mean ± SD (%)	Replicate A (# / %)	Replicate B (# / %)	Replicate C (# / %)	Replicate D (# / %)
0 (Neg./Lab. Control)	0 ± 0	0 / 0	0 / 0	0 / 0	0 / 0
6.25	0 ± 0	0 / 0	0 / 0	0 / 0	0 / 0
12.5	0 ± 0	0 / 0	0 / 0	0 / 0	0 / 0
25	0 ± 0	0 / 0	0 / 0	0 / 0	0 / 0
50	0 ± 0	0 / 0	0 / 0	0 / 0	0 / 0
100	0 ± 0	0 / 0	0 / 0	0 / 0	0 / 0

 

Table 2. Number and Percentage of Daphnia Immobilised after 48-hr Exposure

Nominal Concentration of Test Item (mg/L)	Mean ± SD (%)	Replicate A (# / %)	Replicate B (# / %)	Replicate C (# / %)	Replicate D (# / %)
0 (Neg./Lab. Control)	0 ± 0	0 / 0	0 / 0	0 / 0	0 / 0
6.25	0 ± 0	0 / 0	0 / 0	0 / 0	0 / 0
12.5	0 ± 0	0 / 0	0 / 0	0 / 0	0 / 0
25	0 ± 0	0 / 0	0 / 0	0 / 0	0 / 0
50	0 ± 0	0 / 0	0 / 0	0 / 0	0 / 0
100	100 ± 0	5 / 100	5 / 100	5 / 100	5 / 100

 

Table 3. Test Endpoint Summary for CJ301

Concentration (mg/L)	CJ301 (mg/L)
	48-hr EC50	LOEC	NOEC
Nominal	70.7	100	50

Validity criteria fulfilled:

yes

Conclusions:

According to OECD 202 test method, the 48-hr EC50 of CJ301 was 70.7mg/L.

Executive summary:

This test using the procedures outlined in the Maxxam Study Plan for TOX1602 which is based on the SOP for the OECD 202 (BBY2SOP-00017) and OECD 202 (OECD, 2004). The results of this OECD 202 test for CJ301 show that all validity criteria were met.

Based on the nominal concentrations of CJ301:

1.     The 48-hr EC50 was 70.7 mg/L with the 95% confidence limits of 50 mg/L and 100mg/L.

2.     The NOEC and LOEC was 50 mg/L and 100 mg/L, respectively.

Description of key information

The 48-hr EC50 of CJ301 was 70.7mg/L for Daphnia magna (OECD TG202).

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

70.7 mg/L

Additional information

The test was considered valid as 0% of control organisms were immobilised and the minimum dissolved oxygen concentration at the end of the test was 9.0-9.1 mg O2/L. All test validity criteria were met. The pH of the test solutions was within the ranges outlined in the OECD 202 test guideline. The measured concentrations of CJ301 were within 80-120% of their nominal concentrations.

Based on the nominal concentrations of CJ301:

1.     The 48-hr EC50 was 70.7 mg/L with the 95% confidence limits of 50 mg/L and 100mg/L.

2.     The NOEC and LOEC was 50 mg/L and 100 mg/L, respectively.