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Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1988
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: well performed OECD study with GLP

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1988
Report date:
1988

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Deviations:
not specified
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
2-hydroxy-1-naphthaldehyde
EC Number:
211-902-0
EC Name:
2-hydroxy-1-naphthaldehyde
Cas Number:
708-06-5
Molecular formula:
C11H8O2
IUPAC Name:
2-hydroxy-1-naphthaldehyde
Details on test material:
Purity: 93.3 %

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no
Details on test solutions:
To improve distribution of the substance, 0.1 ml Tween 80/1 was used

Test organisms

Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
TEST ORGANISM
- Common name: Zebrabärbling
- Strain: Hamilton-Buchanan
- Source: West Aquqrium, 3422 Bad Lauterberg
- Length at study initiation: 2.9 cm +- 0.21 cm
- Feeding during test: No


ACCLIMATION
- Acclimation period: 14 days
- Acclimation conditions: yes
- Type and amount of food: Tetra Min, tetra Werke Melle
- Feeding frequency: twice a day ad libitum

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h

Test conditions

Test temperature:
21.3 - 23.0 °C
pH:
7.2 - 8.3
Dissolved oxygen:
5 - 9.4 mg/L
Nominal and measured concentrations:
nominal:
0, 0*, 0.1, 0.25, 0.35, 0.5, 0.7, 1.0, 10 mg/L
*solvent control with Tween 80/1
Details on test conditions:
TEST SYSTEM
- Test vessel: open glass vessel, 30cm x 22cm x 24cm, standing in a container with water with controlled temperature (22+-1°C), filled with 10 L water
- Aeration: no
- Type of flow-through : static
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates):
- No. of vessels per control (replicates): 5 + 1 (with Tween 80/1)
- No. of vessels per vehicle control (replicates): 7

TEST MEDIUM / WATER PARAMETERS
- Preparation of dilution water: according to ISO/DIS 7346/1

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 1 hours/day
- Light intensity: about 700 Lux

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
2 - 4, 24, 48, 72 96 hours
checked for lethality, behaviour and appearance

Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Duration:
96 h
Dose descriptor:
other: 0 % mortality
Effect conc.:
0 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
96 h
Dose descriptor:
other: 0 % mortality
Effect conc.:
0.1 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
96 d
Dose descriptor:
other: 0 % mortality
Effect conc.:
0.25 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
96 h
Dose descriptor:
other: 0 % mortality
Effect conc.:
0.35 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
96 h
Dose descriptor:
other: 10 % mortality
Effect conc.:
0.5 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
96 h
Dose descriptor:
other: 100 % mortality
Effect conc.:
0.71 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
96 h
Dose descriptor:
other: 100 % mortality
Effect conc.:
1 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
96 h
Dose descriptor:
other: 100 % mortality
Effect conc.:
10 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Results with reference substance (positive control):
not performed

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The LC50 for toxicity to fih is in the range from 0.5 to 0.71 mg/L
Executive summary:

The substance was tested for toxicity to fish according to OECD guideline 203 for a period of 96 hours. Additionally to the solvent controls, the following concentrations were used: 0.1, 0.25, 0.35, 0.5, 1.0 and 10 mg/L. Due to the bad solubility of the substance, Tween 80/1 was used to support the solubility. For each concentration 10 brachydanio rerio were used. In the concentration range from 0.5 to 10 mg/L lethality occured, whereas in the range from 0.1 to 0.35 mg/L only symptoms could be observed.

Based on the test results the following lethality-valued could be defined.

LC0 = 0.35 mg/L; LC50 = 0.5 - 0.71 mg/L; LC100 = 0.71 mg/L

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