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Environmental fate & pathways

Biodegradation in water: screening tests

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Link to relevant study record(s)

Description of key information

Based on the results from ready biodegradability tests and prediction of delta-3-carene showing that all major constituents are readily biodegradable, it can be concluded that Cypress oil is expected to be readily biodegradable.

Key value for chemical safety assessment

Biodegradation in water:
readily biodegradable

Additional information

All major constituents of Cypress oil are considered readily biodegradable, except delta-3-carene (see table below). However, it has been shown, with a similar (in composition) substance (Pine needle oil) that contains delta-3-carene (15%), that once in mixture delta-3-carene does not inhibit the ready biodegradability property of the substance.



Typical composition

Biodegradation results

Reference in IUCLID 5


5 %

Readily biodegradable

(d,l)-limonene, 138-86-3, Biodegradation in water: screening tests, MITI, 1980, RS, W


60 %

Readily biodegradable

alpha-pinene, 80-56-8, Biodegradation in water: screening tests, MITI, 2007, RS, W


 27 %

Not Readily biodegradable

Delta-3-carene, 13466-78-9, Biodegradation in water, screening test, Biowin v4.10, RS, W

Pine needle oil


Readily biodegradable

Biodegradation in water, Pine needle oil, 2013, RS, W



Therefore, based on a Weight of Evidence approach, it can be possible to consider that Cypress oil is readily biodegradable.


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