Registration Dossier
Registration Dossier
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EC number: 283-626-9 | CAS number: 84696-07-1 Extractives and their physically modified derivatives such as tinctures, concretes, absolutes, essential oils, oleoresins, terpenes, terpene-free fractions, distillates, residues, etc., obtained from Cupressus sempervirens, Pinaceae.
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
Description of key information
An algal toxicity study was performed in accordance with OECD Guideline No 201 and GLP. Under test conditions, the 48h-ErC50 of CYPRESS OIL for Pseudokirchneriella subcapitata was determined to 9.129 mg/L (loading) a (based on growth rate).
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 9.129 mg/L
Additional information
An algal toxicity study was performed in accordance with OECD Guideline No 201 and GLP. Following a preliminary range-finding test, Pseudokirchneriella subcapitata algae were exposed to CYPRESS OIL at nominal loading concentrations of 1.0, 1.6, 2.5, 4.0, 6.3, 10 mg test item/L (three replicate flasks per test concentration and six replicates for control) for 72 h, under constant illumination and shaking at a temperature of 21-25°C. Measurements of biomass, growth inhibition and yield were done by determination of cell densities at 24, 48 and 72 h using a counting chamber. The results obtained with positive control (potassium chromate) were in accordance with guideline requirements.
The concentrations of the test item (with and without algae) were measured at the start of the study and at the 72-h incubation period.The loss of each relevant constituent was identified in this study and differences occurred due to their physico-chemical properties and behaviour in aqueous solution as part of a mixture, thus the ratio of certain constituents may have changed over time. Since the study was carried out using WAFs made of several constituents with different stability during testing, it was considered acceptable to base the results on the nominal loading values
Under the experimental conditions, the 48-hour EC50for the parameters growth rate and yield were determined to be 9.129 mg test item.L-1(loading) and 2.09 mg test item.L-1(loading), respectively.
Based on the results of this study, CYPRESS OIL is not classified as acute 1 for environment. That study is considered acceptable for that endpoint.
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