Registration Dossier
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EC number: 304-661-9 | CAS number: 94277-77-7
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
Description of key information
With high probability acutely not harmful to aquatic invertebrates.
Key value for chemical safety assessment
Additional information
Key study:
In the acute immobilization test with Daphnia magna the effects of the water soluble fraction (WSF) of the water accommodated fraction (WAF) with a nominal loading of 100 mg/L of the test substance were determined according to OECD guideline 202 [Dr. U. Noack-Laboratorien, 2015]. The limit test was conducted under static conditions over a period of 48 h. At test termination, no effects were observed on Daphnia magna.
Disregarding study:
The short-term toxicity to aquatic invertebrates was determined in a study according to EU C.2 and in compliance with GLP criteria (BASF, 1997).
For the preparation of the stock solution (100 mg/L) the test substance was stirred for about 20 h at 22 ± 1 °C in M4-Medium. Undissolved test substance was tried to remove by paper flltration and centrifugation for about 20 min at about 6000 rpm. It turned out that the paper filter was not the right filter to remove the undissolved particles from the solution (!).
In this study groups of 20 dapnids (D. magna; 4 replicates of 5 animals) were exposed to nominal loading rates of 0 (control), 1.56, 3.13, 6.25, 12.5, 25, 50 and 100 mg/L for 48 hours under static conditions. The nominal test solutions of 1.56, 12.5 and 100 mg/L and the blank control were analytically verified at the start and at the end of the exposure period. The measured concentrations were below the detection limit in all analysed test solutions. Therefore, effect concentrations are expressed as nominal. Observations for swimming ability (mobility) were made at 0, 3, 6 24 and 48 h after the start of exposure. Based on these findings, the 48 -h EC50 was determined at 65.3 mg/L, higher than the solubility limit of the test substance in water. The study is not reliable, because undissolved particles weren't remove from the solution. Therefore, a new study were performed (see key study).
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