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EC number: 943-043-9 | CAS number: 8015-91-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 18/09/2014 - 29/05/2015
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Study according to international guideline (OECD guideline 202) under GLP. No deviations from guideline.
Cross-reference
- Reason / purpose for cross-reference:
- reference to same study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 015
- Report date:
- 2015
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- Principles of method if other than guideline:
- Not relevant
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Essential oil of Cinnamon bark obtained from the bark of Cinnamomum zeylanicum, Lauraceae by distillation
- Cas Number:
- 8015-91-6
- IUPAC Name:
- Essential oil of Cinnamon bark obtained from the bark of Cinnamomum zeylanicum, Lauraceae by distillation
- Test material form:
- other: Liquid
- Details on test material:
- - Name of test material (as cited in study report): Cinnamon bark oil
- Physical state: liquid
1
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
Not relevant
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: water accomodated fractions (WAF) with nominal loading rates of 0.291, 0.64, 1.41, 3.10, 6.82 and 15.0 mg/l
- Sampling method: Analytical samples were taken from each test item concentration and control at 0 hours (initial value) and after 48 hours from aged test solutions in the main test.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Due to the low water solubility of the test item, test item solutions (≥ 1.00 mg/L) were prepared by directly weighing of each “loading rate” (concentration) (15.0, 6.82, 3.10, 1.41 mg/L) into test medium. These solutions were stirred by a magnetic stirrer in a closed vessel at room temperature in the dark over 23 hours to dissolve a maximum amount of the test item. Subsequently the undissolved test item was allowed to sediment and/or float for a period of at least one hour or until the phases have separated. The test item solutions were withdrawn from the middle of the preparation vessel by suction (according to OECD Series on Testing and Assessment No. 23). Transfer of undissolved test item was avoided. This was checked by microscopic observation. To prepare test solutions with test item concentrations of 0.640 and 0.291 mg/L, adequate aliquots of the 15 mg/L withdrawn phase were diluted with test medium.
- Controls: yes, blanks
- Evidence of undissolved material (e.g. precipitate, surface film, etc): Transfer of undissolved test item was avoided. This was checked by microscopic observation.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna
- Strain: Straus, Clone V
- Source: Umweltbundesamt in Berlin/Germany
- Age at study initiation (mean and range, SD): Less than 24 hours
- Method of breeding: Daphnia magna was reared as single culture where one daphnid is kept per 100 mL Elendt M4 medium. The pH-value of the aerated water was within a range of 6.0 – 9.0. The dissolved oxygen was above 60 % saturation and the total hardness 140 - 250 mg/L (as CaCO3). The animals were fed with single cell green algae (Desmodesmus subspicatus, formerly Scenedesmus subspicatus) at least three times a week. The daphnids were reared at a temperature of 20 ± 2 °C in a climatic chamber with 16 hours of illumination and 8 hours of darkness. The medium was changed three times per week. A pipette was used to separate the young daphnids from the adults.
- Feeding during test: No
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Post exposure observation period:
- Not relevant
Test conditions
- Hardness:
- 14°dH corresponding to 250 mg/L CaCO3
- Test temperature:
- 20.3 – 21.7 °C
- pH:
- 7.28 – 7.76
- Dissolved oxygen:
- 8.0 mg/L ± 0.4 mg/L
- Salinity:
- Not relevant
- Nominal and measured concentrations:
- Nominal: 0.291, 0.64, 1.41, 3.10, 6.82 and 15.0 mg/l (WAF)
Measured (t=0h, fresh): < LoQ,Measured (t=48h, aged): - Details on test conditions:
- TEST SYSTEM
- Test vessel: Bottle
- Type (delete if not applicable): closed
- Material, size, headspace, fill volume: Glass, 100 ml, filled with 100 ml test medium and minimum headspace
- Aeration: none
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Elendt M4 medium
- Culture medium different from test medium: No
- Intervals of water quality measurement: The test temperature and the pH-value as well as the oxygen concentration of the test media were measured at all concentrations at t = 0 and 24 h from fresh test solutions and at 24 and 48 h from aged test solutions in one additional replicate per loading rate and control in the non-GLP range finding test and the main test.
OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: No exposure to light
EFFECT PARAMETERS MEASURED (with observation intervals if applicable): immobility (at 24 and 28 hours)
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2.2
- Range finding study
- Test concentrations: 1.0, 10.0 and 100 mg/l (WAF)
- Results used to determine the conditions for the definitive study: yes- Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 24 h
- Dose descriptor:
- EL50
- Effect conc.:
- > 15 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EL50
- Effect conc.:
- 14.2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 95% CL = 13.0 - 15.6 mg/l
- Details on results:
- - Mortality of control: 0%
- Results with reference substance (positive control):
- - Results with reference substance valid? yes
- Mortality: 100%
- EC50/LC50: 1 mg/l < 24h-EC50 < 2.0 mg/l - Reported statistics and error estimates:
- The 24 h and 48 h EL50 are the estimated loading rate where 50 % of the daphnids were immobilised after 24 and 48 hours, respectively. As the immobility at the highest loading rate was only at 25 % after 24 hours, no statistical evaluation was indicated. For the evaluation of the 48 h EL50, probit analysis following Gompertz procedure was used. The evaluation of data was performed by using SAS® Proprietary Software Version 9.3. The NOEL was established based on the highest loading rate at which the immobilisation is not higher than the allowed control immobilisation (=< 10 % immobilisation).
Any other information on results incl. tables
Immobilisation after 24 hours
Test item loading rate (mg/l) |
Control |
0.291 |
0.640 |
1.41 |
3.10 |
6.82 |
15.0 |
|
Immobilised daphnids after 24h |
||||||
Group 1 |
0 |
0 |
0 |
0 |
0 |
0 |
2 |
Group 2 |
0 |
0 |
0 |
0 |
0 |
0 |
1 |
Group 3 |
0 |
0 |
0 |
0 |
0 |
0 |
1 |
Group 4 |
0 |
0 |
0 |
0 |
0 |
0 |
1 |
Sum |
0 |
0 |
0 |
0 |
0 |
0 |
5 |
Percentage (%) |
0 |
0 |
0 |
0 |
0 |
0 |
25 |
Immobilisation after 48 hours
Test item loading rate (mg/l) |
Control |
0.291 |
0.640 |
1.41 |
3.10 |
6.82 |
15.0 |
|
Immobilised daphnids after 48h |
||||||
Group 1 |
0 |
0 |
0 |
0 |
0 |
1 |
3 |
Group 2 |
0 |
0 |
0 |
0 |
0 |
0 |
3 |
Group 3 |
0 |
0 |
0 |
0 |
0 |
1 |
3 |
Group 4 |
0 |
0 |
0 |
0 |
0 |
0 |
2 |
Sum |
0 |
0 |
0 |
0 |
0 |
2 |
11 |
Percentage (%) |
0 |
0 |
0 |
0 |
0 |
10 |
55 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- Less than 10% mortality in controls, Oxygen concentration above 3 mg/l
- Conclusions:
- The acute toxicity (48h-EL50) of Cinnamon bark oil towards Daphnia magna is 14.2 mg/l.
- Executive summary:
The acute toxicity of Cinnamon bark oil towards Dahnia magna was investigated according to OECD guideline 202 under GLP. Daphnids were exposed to WAFs with nominal loading rates of 0.291, 0.640, 1.41, 3.10, 6.82 and 15.0 mg/l and were observed for 48 hours. Based on nominal loading rates, the 24h-EL50 and 48h-EL50 were found to be >15.0 and 14.2 mg/l respectively.
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