A mixture of: 3,3'-dicyclohexyl-1,1'-methylenebis(4,1-phenylene)diurea; 3-cyclohexyl-1-(4-(4-(3-octadecylureido)benzyl)phenyl)urea; 3,3'-dioctadecyl-1,1'-methylenebis(4,1-phenylene)diurea

Short-term toxicity to fish

Description of key information

The 96-h LC50 value in fish is higher than the limit of solubility in test medium at a nominal loading rate of >1000 mg/L.

Additional information

The short-term toxicity to fish was examined in a study according to OECD TG 201 and in compliance with GLP criteria. Groups of Zebrafish (3 replicates of 10 animals) were exposed to a supersaturated solution prepared at a loading rate of 1000 mg/L for 96 hours under static conditions. In a range finding study performed with a saturated solution (1000 mg/L, filtered) and a supersaturated solution (1000 mg/L, unfiltered) no effects on fish were observed. Therefore, the final test was performed as a limit test with the supersaturated (unfiltered) solution in which test substance particles were visibly dispersed through the medium, on the bottom and floating on the surface. During the total 96-hour exposure period no mortality of fish or any other effects were observed in the supersaturated solutions or the blank. No test substance analysis was considered because the test substance was insoluble in water and zero effects were seen after exposure to the supersaturated solution. This is considered to be acceptable as in later aquatic toxicity studies it was found that no adequate analytical method is available for the substance. Based on the findings in the test, the 96 -h LC50 value is higher than the limit of solubility in test medium at a nominal loading rate of >1000 mg/L.