Registration Dossier

Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
migrated information: read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP-Guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1994
Report date:
1994

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
2-hexyloxyethanol
EC Number:
203-951-1
EC Name:
2-hexyloxyethanol
Cas Number:
112-25-4
Molecular formula:
C8H18O2
IUPAC Name:
2-(hexyloxy)ethanol
Details on test material:
- Name of test material (as cited in study report): N-Hexylglykol
- Analytical purity: 98.23%
- Purity test date: 27 Jun 1991
- Substance No.: 91/238
- Lot/batch No.: Tank B 773
- Physical state: liquid

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
TEST ORGANISM
- Common name: Zebra fish
- Source: West Aquarium GmbH, Bad Lauterberg, Germany
- Age at study initiation (mean and range, SD): about 4 month
- Length at study initiation (length definition, mean, range and SD): 2.9 cm (range: 2.7 - 3.1)
- Weight at study initiation (mean and range, SD): 0.233 g (range 0.17 - 0.30)
- Feeding during test
- Food type: "Tetra Min" standard feed for aquarium fish Tetra - Werke, Melle, Germany
- Amount: ad libitum
- Frequency: 1 day before and during exposure


ACCLIMATION
- Acclimation period: none
- Acclimation conditions (same as test or not): same
- Health during acclimation (any mortality observed): none

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h

Test conditions

Hardness:
about 2.5 mmol/L (about 250 mg/l CaCO3)
Test temperature:
23 °C
pH:
about 8.0 - 8.6
Dissolved oxygen:
7.7 - 8.2
Nominal and measured concentrations:
Nominal concentrations: 0, 46.4, 100.0, 215.0, 464.0 and 1000.0 mg/L
Analytical concentrations after 0 h: 0, 39.5, 88.0, 206.0, 469.0 and 1026.0 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: aquaria
- Type: open
- Material, size, fill volume: glass, 30 cm x 22 cm x 24 cm, 10 L
- Aeration: slight
- No. of organisms per vessel: 10
- Biomass loading rate: 0.23


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: municipal water of the city of Frankenthal, not chlorinated and passed through an active carbon filter, aerated

OTHER TEST CONDITIONS
- Photoperiod: 16 : 8 day-night regime

EFFECT PARAMETERS MEASURED:
mortality and symptoms of intoxication

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2.2
- Range finding study
- Results used to determine the conditions for the definitive study: LC 50 after 96 h: between 100 and 1000 mg/L

Preparation of the test solutions:
Separately for each concentration test substance were added to the test water. Subsequently the fish were placed into the aquaria within 25 min after the test material was added.

Results and discussion

Effect concentrationsopen allclose all
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 100 - 220 mg/L
Nominal / measured:
nominal
Basis for effect:
mortality (fish)
Duration:
96 h
Dose descriptor:
NOEC
Effect conc.:
46 mg/L
Nominal / measured:
nominal
Basis for effect:
mortality (fish)
Duration:
48 h
Dose descriptor:
LC0
Effect conc.:
100 mg/L
Nominal / measured:
nominal
Basis for effect:
mortality (fish)
Duration:
96 h
Dose descriptor:
LC100
Effect conc.:
215 mg/L
Nominal / measured:
nominal
Basis for effect:
mortality (fish)

Any other information on results incl. tables

Sublethal observations / clinical signs:

Cumulative mortality after x hours

 Nominal concentration (mg/L)  1  24  48  72  96
 0  0
 46.4  0
 100.0  0 0
 215.0  10 10  10  10  10  10 
 464.0  10 10 10 10  10  10 
 1000.0  10 10  10  10  10  10 

Symptoms of intoxication after x hours

Nominal concentration (mg/L)  1  24  48  72  96
 0  - - - -
 46.4  - - - -
 100.0 A A (A)  (A) (A) (A)

Applicant's summary and conclusion