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Environmental fate & pathways

Biodegradation in water: screening tests

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Description of key information

The ready biodegradability of the test substance was investigated according to the OECD 301F (1992). The test substance undergoes 83 % biodegradation after 28 days. The test substance is regarded as readily biodegradable according to OECD guideline.

Key value for chemical safety assessment

Biodegradation in water:
readily biodegradable

Additional information

The ready biodegradability of the test substance was investigated according to the OECD 301F (1992). The consumption of oxygen was determined by measuring the pressure drop in the respirometer flask over 55 days. As inoculum fresh activated sludge from a biological waste water treatment plant treating predominantly domestic sewage was used. The initial concentration of the test substance was 30 mg/L and the concentration of the active sludge was 6.94 g/L (dry weight of suspended solids) and 2.62 g/L (diluted). The test substance was considered to be non-toxic to the microorganisms at the test concentration. As a reference compound 100 mg/L of sodium benzoate was tested. The degradation of the reference substance exceeded 40 % after 7 days and 65 % after 14 days. The validity criteria were met. The test substance undergoes 83% biodegradation after 28 days and 86 % biodegradation after 55 days in the test conditions. The 10-day window criterion was also fulfilled (11 % biodegradation on day 1 and 77 % on day 11). Thus, the test substance is regarded as readily biodegradable according to OECD guideline.