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Environmental fate & pathways

Biodegradation in water: screening tests

Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
06-05-2002 to 21-05-2002
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP guideline study (OECD), validity criteria fulfilled

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2002
Report date:
2002

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 301 A (Ready Biodegradability: DOC Die Away Test)
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
L-2-hydroxy-2-phenylacetic acid
EC Number:
210-276-6
EC Name:
L-2-hydroxy-2-phenylacetic acid
Cas Number:
611-71-2
Molecular formula:
C8H8O3
IUPAC Name:
hydroxy(phenyl)acetic acid
Details on test material:
ChiPros (R)-Mandelsäure: (R)-mandelic acid (purity: 100 %)

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, domestic, non-adapted
Duration of test (contact time):
14 d
Initial test substance concentrationopen allclose all
Initial conc.:
20 mg/L
Based on:
DOC
Initial conc.:
32 mg/L
Based on:
test mat.
Reference substance
Reference substance:
aniline

Results and discussion

% Degradation
Value:
ca. 90 - ca. 100
Sampling time:
14 d
Details on results:
RS-Freetext:
DOC (mg/l, average values):
time BC RS IH PC AC TS
(d)
0 0.3 20.0 41.3 21.5 21.6 21.4
1 0.3 20.6 41.5 21.4 22.0 21.4
3 1.9 18.2 20.3 21.7 22.2 2.4
5 0.5 2.1 2.9 21.3 21.9 1.2
7 1.6 2.6 3.0 21.9 1.5
10 0.8 2.5 2.9 21.6 1.1
13 1.1 2.6 2.4 22.0 1.1
14 0.8 2.2 2.0 22.0 1.2

DOC decrease (%):
time RS IH PC AC TS
(d)
0 0 0 0 0 0
1 -3 0 0 -1 -2
3 17 55 -1 5 100
5 92 94 1 0 97
7 95 97 -2 100
10 91 95 0 99
13 92 97 -2 100
14 93 97 -2 99


BC = blank control
RS = reference substance
IH = inhibition control
PC = abiotic control
AC = adsorption control
TS = test substance

- adaptation phase: 1-2 days

- degradation phase: 1-2 days

- 10-day window met: yes

Kinetic of test substance (in %):
< 0 after 1 day(s)
ca. 100 after 3 day(s)
ca. 100 after 7 day(s)
ca. 99 after 14 day(s)
Kinetic of control substance (in %):
ca. 90 ... 100 after 14 day(s)
Degradation products: not measured

Any other information on results incl. tables


preliminary investigations (non-GLP):

- TOC = 627 mg/g

- DOC = 634 mg/g

Applicant's summary and conclusion

Interpretation of results:
readily biodegradable