Benzyl propionate

Dermal absorption

Administrative data

Endpoint:

dermal absorption in vitro / ex vivo

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference

Reference Type:

publication

Title:

PENETRATION OF FRAGRANCE COMPOUNDS THROUGH HUMAN EPIDERMIS

Author:

Yuko Jimbo

Year:

1983

Bibliographic source:

The Journal of Dermatology Vol. 10: 229 - 239, 1983

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

Penetration through excised human epidermis was measured using glass chamber followed by gas chromatography.

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Benzyl propionate

EC Number:

204-559-3

EC Name:

Benzyl propionate

Cas Number:

122-63-4

Molecular formula:

C10H12O2

IUPAC Name:

benzyl propanoate

Radiolabelling:

no

Test animals

Details on test animals or test system and environmental conditions:

Not applicable.

Administration / exposure

Details on in vitro test system (if applicable):

SKIN PREPARATION
- Source of skin: Human skin was excised from a cadaver during autopsy, kept at -20°C and thawed prior to examination.
- Type of skin: lower abdominal skin
- Preparative technique: Subcutaneous tissue was removed using Cooper's scissors. Epidermis was separated from dermis by a modification of Baumberger's method. Full thickness skin was placed dermis side down on a metal plate heated to 60°C, for a period of 10 minutes. The epidermis was separated from the dermis using forceps.

PRINCIPLES OF ASSAY
- Glass chamber: The upper surface of the epidermis was fixed to the lowest part of the glass tube, using adhesive. The glass tube was then placed inside one arm of the U-shaped glass Chamber. The glass tube was withdrawn from the other side of the U-shaped glass Chamber, and approximately 5 ml of saline was poured into the Chamber until it came into complete contact with the bottom of the epidermis. The glass tube was then replaced into the Chamber.

- Application of test samples: 0.2 mL of each test sample was applied to the top of the epidermis attached to the glass tube by using a micropipette. In order to avoid evaporation of the test sample, the mouth of the glass tube was covered with Parafilm (American Can Company).
The Chamber was kept in a thermostatically controlied cabinet (HAM-40 type, Seiwa Riko Company) at 21°C and 55% relative humidity for 72 hours.

- Measurements of penetration: Seventy-two hours after application of each test sample, the glass tube was removed. The saline from the U-shaped Chamber was poured into a test tube. The U-shaped Chamber and the bottom of the epidermis attached to the glass tube were both washed 3 times with saline which was also poured into the same test tube. The final volume in the test tube of both the original saline and that used for washing was approximately 10 mL. This saline was then poured into a 100 mL flask. 10 mL of saturated salt water and 25 mL of ether were added to the flask and mixed vigorously. The Compound was extracted in ether. Furthermore, an additional 25 mL of ether was added to the water fraction remaining after extraction, in order to insure complete extraction. The resultant 50 mL of ether, after the two extractions, was dehydrated by adding approximately 2 grams of anhydrous Na2SO4. In order to remove the anhydrous Na2SO4, the ether fraction was filtered with filter paper (Toyo Filter Paper No. 2) and then condensed to 1 mL of ether by using a Kderna-Danish condenser. 2 µL of the condensed sample was injected into a Shimazu GC-6A gas Chromatograph. The peak area on the gas chromatograms was compared with that of a Standard sample, in which the concentration of the material tested was known.

Results and discussion

Absorption in different matrices:

- Skin preparation (in vitro test system): 0.392% (+/- 0.036%) of the test amount penetrated human epidermis

Applicant's summary and conclusion

Conclusions:

As a result, 0.392% of the test amount penetrated human epidermis skin.

Executive summary:

Penetration through excised human epidermis skin of the test item was examined using a glass chamber system. An appropriate amount of test sample was applied for 72 hours to the top of the epidermis attached to the glass tube. The amount of the compound was determined by gas chromatography. As a result, 0.392% of the test amount penetrated human epidermis skin.