Reaction mass of 1,4-dichlorobut-2-ene; 3,4-dichlorobut-1-ene

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

other information

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Adopted according to OECD SIDS (public available peer reviewed source). The original source is not available and has not been reviewed.

Data source

Reference

Reference 1

Reference Type:

review article or handbook

Title:

1,4-Dichlorobut-2-ene- CAS No: 764-41-0

Author:

OECD SIDS

Year:

2006

Bibliographic source:

SIDS Initial Assessment Report for 22th SIAM, UNEP Publications

Reference 2

Reference Type:

study report

Title:

Unnamed

Year:

1974

Report date:

1974

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

The LD50 of a mixture of 1.43 % cis- and 97.17% trans-isomer has been determined in ChR-CD rats after a 30 minutes exposure.

GLP compliance:

no

Remarks:

GLP was not mandatory at the time of the study

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

1,4-dichlorobut-2-ene

EC Number:

212-121-8

EC Name:

1,4-dichlorobut-2-ene

Cas Number:

764-41-0

Molecular formula:

C4H6Cl2

IUPAC Name:

1,4-dichlorobut-2-ene

Details on test material:

1.43 % cis- and 97.17% trans-1,4-dichlorobut-2-ene.

Test animals

Species:

rat

Strain:

other: ChR-CD

Sex:

male

Administration / exposure

Route of administration:

inhalation: vapour

Type of inhalation exposure:

whole body

Vehicle:

other: filtered air

Analytical verification of test atmosphere concentrations:

not specified

Duration of exposure:

30 min

Concentrations:

240/370/410/440/540/760/2600 ppm

No. of animals per sex per dose:

6 (only male rats)

Control animals:

other: not required

Details on study design:

- Duration of observation period following administration: 14 days
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, histopathology, gross examination. Gross and histopathologic examinations were performed on 2 rats surviving exposure for 7 days, on 1 rat found dead after 13 recovery days and on 1 rat after 14 recovery days.

Statistics:

LC50 calculations were made by a computerized version of the probit method of Finney (1964).

Results and discussion

Effect levels

Sex:

male

Dose descriptor:

LC50

Effect level:

4.1 mg/L air

Based on:

test mat.

Exp. duration:

30 min

Mortality:

Mortality %: 0/0/33/0/0 /83/100

Clinical signs:

other: Clinical signs were observed already in the lowest dose group (240 ppm; 1248 mg/m³): Inactivity, closed eyes, shallow respiration, pale ears, salivation, lacrimation, wet fur and reversible loss of body weight. Clinical signs at 760 ppm: Irregular respira

Body weight:

No data

Gross pathology:

No data

Other findings:

- Histopathology: at 410 ppm and above: damage of the tracheobronchial epithelium was observed. Furthermore in animals exposed to 760 ppm (3950 mg/m³) there was kidney damage, testicular atrophy and hypoplastic bone marrow observed. These effects have been interpreted by the authors of the study as representing only a reflection of stress and emaciation and thus being not directly compound-related. The other tissues examined (lymph node, stomach, duodenum, epididymis, thyroid, adrenal gland, brain, eye) revealed no effects being attributable to the test substance.