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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
toxicity to reproduction: other studies
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
other: study using an unulual application route but providing useful information

Data source

Referenceopen allclose all

Reference Type:
publication
Title:
The detection of carcinogen-induced sperm head abnormalities in mice
Author:
Topham JC
Year:
1980
Bibliographic source:
Mutat Res 69, 149-155 (1980)
Reference Type:
review article or handbook
Title:
BUA report 140: 2,4-Dichloranilin; 2,5-Dichloranilin; 3,4-Dichloranilin.
Author:
Beratergremium für umweltrelevante Altstoffe (BUA).
Year:
1994
Bibliographic source:
S Hirzel, Wissenschaftliche Verlagsgesellschaft, Stuttgart ISBN: 3-7776-0618-9

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
Groups of 5 male rats received on 5 days i.p. injections of 25, 50, 100, 250 and 500 mg/kg bw 2,4-dichloroaniline dissolved in corn oil. After the 5 week observation period surviving animals were sacrificed and sperm morphology was examined.
GLP compliance:
not specified
Type of method:
other: ex vivo

Test material

Constituent 1
Chemical structure
Reference substance name:
2,4-dichloroaniline
EC Number:
209-057-8
EC Name:
2,4-dichloroaniline
Cas Number:
554-00-7
Molecular formula:
C6H5Cl2N
IUPAC Name:
2,4-dichloroaniline
Test material form:
other: solid
Details on test material:
no data

Test animals

Species:
mouse
Strain:
other: F1-Generation from male CBA and female BALB/s mice
Sex:
male
Details on test animals or test system and environmental conditions:
no data

Administration / exposure

Route of administration:
intraperitoneal
Vehicle:
corn oil
Details on exposure:
Groups of 5 male rats received on 5 days i.p. injections of 25, 50, 100, 250 and 500 mg/kg bw 2,4-dichloroaniline dissolved in corn oil. After the 5 week observation period surviving animals were sacrificed and sperm morphology was examined.
Analytical verification of doses or concentrations:
no
Duration of treatment / exposure:
once per day on 5 days
Frequency of treatment:
once per day on 5 days
Duration of test:
an observation period of 5 weeks followed after the last injection
No. of animals per sex per dose:
5 males
Control animals:
yes, concurrent vehicle
Details on study design:
Groups of 5 male rats received on 5 days i.p. injections of 25, 50, 100, 250 and 500 mg/kg bw 2,4-dichloroaniline dissolved in corn oil. After the 5 week observation period surviving animals were sacrificed and sperm morphology was examined.
Statistics:
no data

Results and discussion

Effect levels

open allclose all
Remarks on result:
not measured/tested
Remarks:
Effect level not specified (migrated information)
Dose descriptor:
other: NOAEL (sperm head abnormality)
Effect level:
ca. 500 mg/kg bw/day (actual dose received)

Observed effects

mortality occurred in the 500 mg7kg bw group

Applicant's summary and conclusion

Executive summary:

Groups of 5 male rats received on 5 days i.p. injections of 25, 50, 100, 250 and 500 mg/kg bw 2,4-dichloroaniline dissolved in corn oil. After the 5 week observation period surviving animals were sacrificed and sperm morphology was examined. 2,4 -Dichloroaniline did not induce sperm head abnormality in male mice up to 500 mg/kg bw/day