Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 203-013-1 | CAS number: 102-20-5
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
Description of key information
The acute toxicity of the test item to danio rerio was determined according to OECD guideline 203. The LC50 value after 96 h was determined to be 3.65 mg/L.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 3.65 mg/L
Additional information
The acute toxicity of the test item to fish (zebrafish) was determined according to the principles of the OECD-Guideline for Testing of Chemicals No. 203 (1992) and EU Council Regulation (EC) No. 440/2008/ Method C.1 (2008) from 2016-12-12 to 2017-02-03. An acute toxicity test under semi-static conditions with daily renewal of the test media was conducted with the nominal test item concentrations of 0.313 - 0.625 - 1.25 - 2.50 - 5.00 mg/L (factor 2) corresponding to geometric mean measured concentrations (based on the test item and the metabolite Phenylethylalcohol) of 0.247 - 0.487 - 1.28 - 2.58 - 4.22 mg/L. Duration of the test was 96 hours. Seven test organisms were exposed to each test concentration and the control. Water quality parameters (temperature, pH-value and oxygen-saturation) measured at 0, 24, 48, 72 and 96 hours were determined to be within the acceptable limits.
The concentrations of the test item were analytically verified via HPLC-DAD at the start (0, 24, 48, 72 h) and at the end (24, 48, 72, 96 h) of the exposure intervals in all tested concentration levels and the control. The concentrations of the test item decreased during the exposure, because of hydrolyses. Therefore one degradation product was analytically verified in all aged test item concentration levels and the control. These results were calculated with the hydrolysis factor (1.967) to the test item and summed up with the measured concentration of the test item. The measured concentrations at the start of all exposure intervals after 0 to 72 hours were between 80 and 150 % of the nominal values. At the end of all exposure intervals after 24 to 96 hours the measured concentrations of the test item were between 48 to 129 %. These results led to the geometric mean measured concentrations (based on the test item and the metabolite) 0.247 - 0.487 - 1.28 - 2.58 - 4.22 mg/L test item, which all effects were based on. All validity criteria of the test guideline were met. The LC50 value of the test item after 96h was 3.65 mg/L.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
