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Ecotoxicological information

Short-term toxicity to fish

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Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: The study was conducted according to O.E.C.D. Testing Guideline No. 203 with GLP compliance.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2010
Report Date:
2010

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Deviations:
not specified
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent
Type:
Constituent
Type:
Constituent
Type:
Constituent
Details on test material:
As per IUCLID5 Sections 1.1. -1.4.

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
Test solutions were sampled for BPA-Tars concentration determination at 0 and 96 hr.

Test solutions

Vehicle:
yes
Details on test solutions:
Required amounts of BPA-Tars were added to 5 litres of dilution water and treated for one hour in an ultrasonic bath and afterwards stirred for 24 h on magnetic stirrers. The nominal concentrations were: 0, 6.25, 12.5, 25, 50 and 100 mg/L.

Test organisms

Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
Zebra fish (Danio rerio) were acquyired from Interaquaristik (Germany). The fish were maintained at 20-24 °C with a dissolved oxygen content of > 5 mg/L and feed commercial fish food, daily. upto 24 h prior to test initiation.

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Post exposure observation period:
None

Test conditions

Hardness:
14.8°dH (= 264 mg/L CaCO3).
Test temperature:
21.9 - 22.7 degrees C.
pH:
7.5 - 7.9
Dissolved oxygen:
The dissolved oxygen concentration remained above 60% of the air-saturation value throughout the exposure period.
Salinity:
no data, freshwater
Nominal and measured concentrations:
Nominal concentrations were: 6.25, 12.5, 25, 50 and 100 mg/L and the measured concentrations ranged from 25.9% - 28.1% of the nominal concentration.
Details on test conditions:
Fish were exposed in glass aquaria holding 5 L of test media covered with glass plates with 7 animals per test concentration. there was no feeding during the exposure period of 96 hr. Exposure was by a static system with gentle aeration via narrow glass tubes.
Reference substance (positive control):
no

Results and discussion

Effect concentrations
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
4.95 mg/L
Nominal / measured:
meas. (initial)
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: the LC50 was 4.95 mg/L from 24 hr to 96 hr.
Details on results:
The initial LC50 value at two hr exposure was 19.8 mg/L. The final LC50 value was 4.95 mg/L after 24 hr of exposure
Results with reference substance (positive control):
No data
Reported statistics and error estimates:
The LC 50 was calculated by probit analysis using statistic programm ToxRat Professional version 2.10.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
BPA-Tars was demonstrated to have an acute LC50 for the Zebrafish of 4.95 mg/L from 24 to 96 hr of exposure. This finding suggest that BPA-Tars requires Classification and Labeling as Toxic to aquatic organisms.
Executive summary:

2 -Acetone polymer with phenol (BPA-Tars) was assessed for acute toxicity to the Zebrafish by an O.E.C.D. Testing Guideline No. 203 study with GLP comlpliance. BPA-Tars was demonstrated to have an acute LC50 for the Zebrafish of 4.95 mg/L from 24 to 96 hr of exposure. This finding suggest that BPA-Tars requires Classification and Labeling as Toxic to aquatic organisms.