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Short-term toxicity to aquatic invertebrates

Description of key information

OECD Guideline 202, EU Method C.2, GLP, key study, validity 2:
48h-EC50 = 15 mg/L (95% CL: 10 - 19 mg/L; based on time-weighted mean measured test concentrations). 

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

15 mg/L

Additional information

One key study is available to assess the acute toxicity of the substance to Daphnia magna. This study was performed according to OECD Guideline 202 and EU Method C.2 with GLP statement.

Following preliminary range-finding tests and an initial experiment, twenty daphnids (2 replicates of 10 animals) were exposed to an aqueous solution of the test material at concentrations of 0.282, 0.529, 0.889, 1.52, 2.55, 4.47, 7.87, 13.8 and 22.6 mg/l (time-weighted mean measured concentrations) for 48 hours at a temperature of 21 °C to 22°C under static test conditions. The test material solutions were prepared by shaking an excess (1000 mg/l) of test material in reconstituted water using an INFORS Aerotron shaker at 300 rpm at a temperature of 30°C for 24 hours prior to cooling to approximately 20°C and removal of any undissolved test material by filtration through a 0.2µm Gelman Acrocap filter (first approximate 100 ml discarded to pre-condition the filter) to produce a saturated solution of 100% v/v. Dilutions were made from this saturated solution to prepare the remainder of the test series. The number of immobilised Daphnia were recorded after 24 and 48 hours.

Analysis of the saturated solution at 0 hours showed a measured concentration of 69 mg/L. Analysis of the test preparations at 0 hours showed measured test concentrations to range from 0.287 to 23.6 mg/l and analysis at 48 hours showed measured concentrations to range from 0.278 to 21.6 mg/l. Inspection of the data indicated that there was a very slight decline in measured concentrations over the 48-Hour test period. It was therefore considered justifiable to base the results on the time-weighted mean measured test concentrations.

The 48-Hour EC50 for the test material to Daphnia magna based on time-weighted mean measured test concentrations was 15 mg/L with 95% confidence limits of 10 - 19 mg/L. The highest concentration without observed effect was 1.5 mg/L.