1H-Pyrazole-5-carboxylic acid, 3-(chloromethyl)-1-(3-chloro-2-pyridinyl)-, methyl ester

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP study according EU/OECD guideline.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2013

Report date:

2013

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 423 (Acute Oral toxicity - Acute Toxic Class Method)

Version / remarks:

(2001)

GLP compliance:

yes (incl. QA statement)

Test type:

acute toxic class method

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

methyl 3-(chloromethyl)-1-(3-chloropyridin-2-yl)-1H-pyrazole-5-carboxylate

EC Number:

936-879-0

Cas Number:

1247712-63-5

Molecular formula:

C11H9Cl2N3O2

IUPAC Name:

methyl 3-(chloromethyl)-1-(3-chloropyridin-2-yl)-1H-pyrazole-5-carboxylate

Test animals

Species:

rat

Strain:

Wistar

Sex:

female

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

other: polyethylene glycol (400)

Doses:

2000 mg/kg body weight (bw)

No. of animals per sex per dose:

six female rats

Control animals:

no

Results and discussion

Effect levels

Sex:

female

Dose descriptor:

LD50

Effect level:

2 500 mg/kg bw

Remarks on result:

other: 1/3 animals died at a initially dose level of 2000 mg/kg bw. No mortalities occurred at the follow-up dose level of 2000 mg/kg bw, therefore, a LD50 cut-off of 2500 mg/kg bw was derived according to OECD TG 423

Applicant's summary and conclusion

Interpretation of results:

not classified

Remarks:

Migrated information Criteria used for interpretation of results: EU

Conclusions:

The test material caused mortality at a dose level of 2000 mg/kg bw (1/6). Clinical signs were decreased activity (1/6), hunched back (3/6), piloerection (3/6), prone position (1/6) and death (1/6). Body weight and body weight gain of the test material treated animals showed no indication of a treatment-related effect. Gritty beige material in the digestive content of the stomach and dark/red discoloured stomach glandular mucosa were found in the dead animal at necropsy and considered to be test item administration-related. Dark/red discoloration of the collapsed lungs was regarded as agonal/post mortem. There was no evidence of the macroscopic observations in surviving animals dosed at 2000 mg/kg bw and terminated on Day 14.