Tetrasodium 8-[[4-[(4-amino-3-sulphonatophenyl)azo]-6-sulphonatonaphthyl]azo]-5-[[6-(benzoylamino)-1-hydroxy-3-sulphonato-2-naphthyl]azo]naphthalene-2-sulphonate

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

28.5. – 14.6.2001

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: meets generally accepted scientific principles, acceptable for assessment

Qualifier:

according to guideline

Guideline:

other: Test No. L 47 "Fish, Acute Toxicity test. Chemical formulations and waste waters."

Deviations:

yes

Remarks:

the dye is precipitated and gradually settled at the bottom

Principles of method if other than guideline:

- Principle of test:A sample of the tested substance was dissolved in the dilution water at a concentration of 0.1 g/L. This solution is initially clear, but during the test, the dye is precipitated and gradually settled at the bottom, in all dilutions.The total volume for one dilution was 1000 ml for preliminary and 4000 ml for limit test. Number of organisms: 3 (preliminary), 10 (limit test)- Short description of test conditions:The dilution water should have a pH of 7.8 and is saturated with oxygen at a temperature 20 – 24°C.Test duration 96 hours, the temperature of the solutions 22.0 ± 1.0 °C.- Parameters analysed / observed: mortality at 48 and 96 h

GLP compliance:

no

Remarks:

Zkušební laboratoř č. 1057, akr. ČIA dle ČSN EN 45001

Specific details on test material used for the study:

Other name: Saturnová námořnická modř LFG 135

Analytical monitoring:

no

Test organisms (species):

Poecilia reticulata

Details on test organisms:

TEST ORGANISM- Common name: Guppy- Strain: Poecilia reticulata- Source: AKVA-SALON, Žižkova 977, 535 01 Přelouč- Age at study initiation (mean and range, SD): 3 - 4 monts- Length at study initiation (length definition, mean, range and SD): 2 - 3 cmACCLIMATION- Acclimation period: yes- Acclimation conditions (same as test or not): same as testFEEDING DURING TESTno feeding during testfasted 72 hours before test

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Test temperature:

22.0 ± 1.0 °C (measured values in attached document with tables)

pH:

7.8 (measured values in attached document with tables)

Dissolved oxygen:

8.0 - 8.5 mg/L O2 (measured values in attached document with tables)

Nominal and measured concentrations:

initial measured concentration 100.1 mg.L-1

Details on test conditions:

TEST SYSTEM- Material, size, headspace, fill volume: open 10 L glass aquarium- Aeration: without- No. of organisms per vessel: Preliminary test: 3 animals Full test: 10 animals - No. of vessels per concentration/control (replicates): no replicatesTEST MEDIUM / WATER PARAMETERSDilution water: standard dilution water was prepared by mixing 2.5 ml of stock solutions (A,B,C,D) with 1000 ml distilled waterA) 117.6 g CaCl3.2H2O in 1L of distilled waterB) 49.3 g MgSO4.7H2O in 1L of distilled waterC) 25.9 g NaHCO3 in 1L of distilled waterD) 2.3 g KCl in 1L of distilled waterThe dilution water should have a pH of 7.8 and is saturated with oxygen at a temperature 20 – 24°C. EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mortality at 48, 96 h TEST CONCENTRATIONS - Range finding study: 100, 75, 50, 25 mg.L-1 - Test concentrations: 100 mg.L-1 - Results used to determine the conditions for the definitive study: see attached document with tables

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Results with reference substance (positive control):

- Results with reference substance valid? Yes

Validity criteria fulfilled:

yes

Remarks:

The measured values of pH solution not change more than 1 pH unit.

Executive summary:

The test substance, Direct Blue 85 , was tested for acute toxicity on fish Poecilia reticulata. The test was performed according to method No. L 47 "Fish, Acute Toxicity test. Chemical formulations and waste waters".

Results:

48 h - LC50 > 100.0 mg.L-1

96 h - LC50 > 100.0 mg.L-1

Description of key information

The test substance was tested for acute toxicity on fish Poecilia reticulata. The test was performed according to method No. L 47 "Fish, Acute Toxicity test. Chemical formulations and waste waters".

Results:

48 h - LC50 > 100.0 mg.L-1

96 h - LC50 > 100.0 mg.L-1

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

100 mg/L