Registration Dossier
Registration Dossier
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 475-300-1 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From April 18, 2006 to April 21, 2006
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)
- Deviations:
- no
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Vehicle:
- no
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Type: mixed population of aquatic microorganisms (activated sludge)
- Origin: aeration tank of a domestic sewage treatment plant (Municipal STP Cologne-Stammheim)
- Pretreatment: aeration of the activated sludge, daily fed with synthetic medium
- pH of the suspension before application: 7.8 - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Test temperature:
- 21.3-21.9°C
- pH:
- Control = 8.1
Test = 7.7-7.9
Reference substance: 8.1 - Dissolved oxygen:
- Control:
Start = 5.0-5.3 mg/L; End = 2.8-3.3 mg/L
Reference substance:
Start = 4.0-6.1 mg/L; End = 2.8-5.4 mg/L
Test:
Start = 3.9-4.3 mg/L; End = 2.7-2.9 mg/L - Nominal and measured concentrations:
- - Type of test material application: direct weighing (The test item was added to about 130 mL of deionized water and stirred overnight before testing)
- Test concentrations (nominal): 100, 1000 and 10,000 mg/L
- Test substance concentration in physico-chemical oxygen consumption control (nominal): 10,000 mg/L
- Concentration of reference substance '3,5-dichlorophenol' (nominal): 5, 10 and 20 mg/L - Details on test conditions:
- - Test concentration of the activated sludge: 1,200 mg/L suspended solids
- Incubation: 3 h with permanent aeration - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol (97% purity; Batch number: 15809K1-392)
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 10 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Key result
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 10 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Results with reference substance (positive control):
- - EC50 for reference substance = 11.0 mg/L
- Reported statistics and error estimates:
- The EC50 value was determined by probit analysis.
- Validity criteria fulfilled:
- yes
- Conclusions:
- Under the test conditions, the NOEC of the test substance was >=10,000 mg/L, indicating a low toxicity to microorganisms.
- Executive summary:
A study was conducted to assess the toxicity of the test substance to sludge microorganisms, according to EU Method C.11 (similar to OECD Guideline 209), in compliance with GLP.
Activated sludge from domestic sewage was exposed to nominal test substance concentrations of 100, 1000 and 10,000 mg/L. The respiration rates of each of these mixture was determined after an aeration period of 3 h and compared to data from the control and the reference substance 3,5-dichlorophenol.
The reference substance produced 23.8, 42.9 and 73.3% inhibition of respiration at 5, 10 and 20 mg/L respectively. The test substance produced 0.6, 0 and 0% inhibition of respiration at concentrations of 100, 1000 and 10,000 mg/L respectively.
Under the test conditions, the EC50 of the test substance was >10,000 mg/L, indicating a low toxicity to microorganisms.
Reference
Table 1: Inhibition of respiration
Treatment group |
Concentration [mg/L] |
Respiratory rate mg/[L×h] |
% inhibition of respiration |
Control |
- |
31.5 |
- |
Test substance |
100 |
31.3* |
0.6 |
Test substance |
1,000 |
32.3* |
0 |
Test substance |
10,000 |
35.3* |
0 |
Reference substance (3, 5 dichlorophenol) |
5 |
24.0 | 23.8 |
Reference substance (3, 5 dichlorophenol) |
10 |
18.0 | 42.9 |
Reference substance (3, 5 dichlorophenol) |
20 |
8.4 | 73.3 |
* Respiratory rate adjusted for physico-chemical oxygen consumption of 0.7 mg/[L×h] at a test substance concentration of 10,000 mg/L.
Description of key information
The NOEC of the test substance was >=10,000 mg/L, indicating a low toxicity to microorganisms.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 10 000 mg/L
- EC10 or NOEC for microorganisms:
- 10 000 mg/L
Additional information
A study was conducted to assess the toxicity of the test substance to sludge microorganisms, according to EU Method C.11 (similar to OECD Guideline 209), in compliance with GLP. Activated sludge from domestic sewage was exposed to nominal test substance concentrations of 100, 1000 and 10,000 mg/L. The respiration rates of each of these mixture was determined after an aeration period of 3 h and compared to data from the control and the reference substance 3,5-dichlorophenol. The reference substance produced 23.8, 42.9 and 73.3% inhibition of respiration at 5, 10 and 20 mg/L respectively. The test substance produced 0.6, 0 and 0% inhibition of respiration at concentrations of 100, 1000 and 10,000 mg/L respectively. Under the test conditions, the EC50 of the test substance was >10,000 mg/L, indicating a low toxicity to microorganisms (Weyers, 2006e).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.

EU Privacy Disclaimer
This website uses cookies to ensure you get the best experience on our websites.