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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
eye irritation: in vitro / ex vivo
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2007

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: An improved CAM Method for Predicting Ocular Irritation, Bagley, D.M., Rizvi, P.Y., Kong, B.M., and De Salva, S.J. (1988), Alternative Methods in Toxicology, Vol. 6, Progess in In Vitro Toxicology, pp. 131-138
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
4-[(1E,3S)-3-ethenyl-3,7-dimethylocta-1,6-dien-1-yl]phenol
EC Number:
685-515-4
Cas Number:
10309-37-2
Molecular formula:
C18H24O
IUPAC Name:
4-[(1E,3S)-3-ethenyl-3,7-dimethylocta-1,6-dien-1-yl]phenol

Test animals / tissue source

Species:
other: chorioallantoic membrane of White Leghorn eggs
Details on test animals or tissues and environmental conditions:
Pre-dose:
The eggs were placed in a commercial incubator at 99 ± 2℉ for 10 days.

Test system

Vehicle:
other: corn oil
Controls:
no
Amount / concentration applied:
concentration: 1%, 5%, 10%, 25%
dose: 40 μl
Duration of treatment / exposure:
30 ± 5 min
Number of animals or in vitro replicates:
10 per concentration

Results and discussion

In vitro

Results
Irritation parameter:
other: RC50
Remarks:
%
Value:
ca. 7.7
Remarks on result:
no indication of irritation

Any other information on results incl. tables

Concentration (%) # of Eggs # of Positive Responses

1.0 10 0/10

5.0 10 2/10

10 10 7/10

25 10 10/10

The positive responses were characterized by capillary injection, hemorrhage and ghost vessels.

The RC50 and 95% Confidence Limits are 7.7 (5.7-10)%

Applicant's summary and conclusion

Interpretation of results:
not irritating
Remarks:
Migrated information Criteria used for interpretation of results: other: RC50 > 3.0% = non-irritant