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Administrative data

Endpoint:
skin irritation: in vivo
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Study meets scientific standards with acceptable restrictions (limited documentation, exposure for 1-15 min and 20h)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1975
Report date:
1975

Materials and methods

Principles of method if other than guideline:
Skin irritation was tested using an internal standard method (BASF test). White Vienna rabbits were used. Usually, 2 animals were treated for 1, 5 and 15 minutes and 2 other animals for 20 hours using occlusive conditions. An application site of 2.5 X 2.5 cm was covered with the liquid test substance.
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
3-dimethylaminopropiononitrile
EC Number:
217-090-4
EC Name:
3-dimethylaminopropiononitrile
Cas Number:
1738-25-6
Molecular formula:
C5H10N2
IUPAC Name:
3-(dimethylamino)propanenitrile
Details on test material:
- Name of test material (as cited in study report): N,N-Dimethyl-amino-propionitril
- Physical state: liquid

Test animals

Species:
rabbit
Strain:
Vienna White

Test system

Type of coverage:
occlusive
Preparation of test site:
shaved
Vehicle:
unchanged (no vehicle)
Controls:
not required
Amount / concentration applied:
ca. 0.5 mL
Duration of treatment / exposure:
Experiment 1: 1, 5, or 15 minutes
Experiment 2: 20 h
Observation period:
Experiment 1: up to 8 days
Experiment 2: up to 8 days
Number of animals:
2 per experiment
Details on study design:
TEST SITE
- Area of exposure: An application site of 2.5 X 2.5 cm was covered with the liquid (37°C; approx. 1 ml)

REMOVAL OF TEST SUBSTANCE
- Washing:
Experiment 1: washing was done in the 1 min, 5 min and 15 min test directly after exposure. Lutrol 50% (mild detergent) was used.
Experiment 2: No washing was done after the 20 h treatment.

SCORING SYSTEM: Descriptive scores of the raw data have been converted to Draize numerical scores

Results and discussion

In vivo

Resultsopen allclose all
Irritation parameter:
erythema score
Basis:
mean
Remarks:
20 h exposure
Time point:
other: 24 - 48 - 72 h
Score:
0.5
Max. score:
4
Reversibility:
fully reversible within: 4 d
Remarks on result:
other: 1/2 animals without any signs of irritation
Irritation parameter:
edema score
Basis:
mean
Remarks:
20 h exposure
Time point:
other: 24 - 48 - 72 h
Score:
0
Max. score:
4
Reversibility:
other: no effects

Any other information on results incl. tables

Exposure to one, five or 15 min caused no erythema or edema.

Applicant's summary and conclusion

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