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Administrative data

Endpoint:
short-term repeated dose toxicity: oral
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
other: Documentation insufficient for complete assessment (missing test parameter). Relevant parameters for repeated dose studies (clinical signs, hematology, clinical signs etc.) were not studied

Data source

Reference
Reference Type:
publication
Title:
STUDIES ON THE MECHANISM OF UROTOXIC EFFECTS OF N,N'-DIMETHYLAMINOPROPIONITRILE IN RATS AND MICE. 1. BIOCHEMICAL AND MORPHOLOGIC CHARACTERIZATION OF THE INJURY AND ITS RELATIONSHIP TO METABOLISM
Author:
Mumtaz M.M. et al.
Year:
1991
Bibliographic source:
J. of Toxicology and Environm. Health 33, 1-17 (1991)

Materials and methods

Principles of method if other than guideline:
14 d study in rats to assess signs of systemic toxicity and effects on target organ of 3-diemthylaminopropionitrile after oral application.
GLP compliance:
not specified
Limit test:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
3-dimethylaminopropiononitrile
EC Number:
217-090-4
EC Name:
3-dimethylaminopropiononitrile
Cas Number:
1738-25-6
Molecular formula:
C5H10N2
IUPAC Name:
3-(dimethylamino)propanenitrile
Details on test material:
The test-substance was obtained from Aldrich Chemical,
Milwaukee.

Test animals

Species:
rat
Strain:
Sprague-Dawley
Sex:
male
Details on test animals or test system and environmental conditions:
TEST ANIMALS
- Source: Timco Laboratories Houston, Texas, USA
- Weight at study initiation: 175 -225 g
- Diet (e.g. ad libitum): ad libitum
- Water (e.g. ad libitum): ad libitum
- Acclimation period: 1 week

ENVIRONMENTAL CONDITIONS
no detailed information

Administration / exposure

Route of administration:
oral: gavage
Vehicle:
not specified
Analytical verification of doses or concentrations:
not specified
Duration of treatment / exposure:
2 weeks
Frequency of treatment:
daily, 5 days per week
Doses / concentrations
Remarks:
Doses / Concentrations:
350 mg/kg bw
Basis:

No. of animals per sex per dose:
5 males
Control animals:
yes
Details on study design:
Post-exposure period: no

Examinations

Observations and examinations performed and frequency:
Animal weight, water consumption, and urine volume were recorded every 24 h .
Sacrifice and pathology:
Morphologic and histologic studies on liver, bladder, and kidney were performed at the end of the 2-wk period.

Results and discussion

Results of examinations

Details on results:
Gradual decrease in body weight between day 6 and 12 and a sharp decrease between day 12-15. Water consumption was slightly but significantly higher than that of control rats during the first 7 days, but decreased sharply thereafter. Comparably, urine volume was found to increase slightly during the first 8 d, followed by a sharp decrease by the end of the experiment.

Effect levels

Dose descriptor:
LOAEL
Effect level:
350 mg/kg bw/day (nominal)
Sex:
male
Basis for effect level:
other: Body weight reduction, reduced water consumption and urine volume

Target system / organ toxicity

Critical effects observed:
not specified

Any other information on results incl. tables

Results of the histological examination were not reported.  

Applicant's summary and conclusion