3-dimethylaminopropiononitrile

Repeated dose toxicity: oral

Administrative data

Endpoint:

short-term repeated dose toxicity: oral

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

other: Documentation insufficient for complete assessment (missing test parameter). Relevant parameters for repeated dose studies (clinical signs, hematology, clinical signs etc.) were not studied

Data source

Reference

Reference Type:

publication

Title:

STUDIES ON THE MECHANISM OF UROTOXIC EFFECTS OF N,N'-DIMETHYLAMINOPROPIONITRILE IN RATS AND MICE. 1. BIOCHEMICAL AND MORPHOLOGIC CHARACTERIZATION OF THE INJURY AND ITS RELATIONSHIP TO METABOLISM

Author:

Mumtaz M.M. et al.

Year:

1991

Bibliographic source:

J. of Toxicology and Environm. Health 33, 1-17 (1991)

Materials and methods

Principles of method if other than guideline:

14 d study in rats to assess signs of systemic toxicity and effects on target organ of 3-diemthylaminopropionitrile after oral application.

GLP compliance:

not specified

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

3-dimethylaminopropiononitrile

EC Number:

217-090-4

EC Name:

3-dimethylaminopropiononitrile

Cas Number:

1738-25-6

Molecular formula:

C5H10N2

IUPAC Name:

3-(dimethylamino)propanenitrile

Details on test material:

The test-substance was obtained from Aldrich Chemical,
Milwaukee.

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

male

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Timco Laboratories Houston, Texas, USA
- Weight at study initiation: 175 -225 g
- Diet (e.g. ad libitum): ad libitum
- Water (e.g. ad libitum): ad libitum
- Acclimation period: 1 week

ENVIRONMENTAL CONDITIONS
no detailed information

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

not specified

Analytical verification of doses or concentrations:

not specified

Duration of treatment / exposure:

2 weeks

Frequency of treatment:

daily, 5 days per week

Doses / concentrations

Remarks:

Doses / Concentrations:
350 mg/kg bw
Basis:

No. of animals per sex per dose:

5 males

Control animals:

yes

Details on study design:

Post-exposure period: no

Examinations

Observations and examinations performed and frequency:

Animal weight, water consumption, and urine volume were recorded every 24 h .

Sacrifice and pathology:

Morphologic and histologic studies on liver, bladder, and kidney were performed at the end of the 2-wk period.

Results and discussion

Results of examinations

Details on results:

Gradual decrease in body weight between day 6 and 12 and a sharp decrease between day 12-15. Water consumption was slightly but significantly higher than that of control rats during the first 7 days, but decreased sharply thereafter. Comparably, urine volume was found to increase slightly during the first 8 d, followed by a sharp decrease by the end of the experiment.

Effect levels

Dose descriptor:

LOAEL

Effect level:

350 mg/kg bw/day (nominal)

Sex:

male

Basis for effect level:

other: Body weight reduction, reduced water consumption and urine volume

Target system / organ toxicity

Critical effects observed:

not specified

Any other information on results incl. tables

Results of the histological examination were not reported.