Registration Dossier

Diss Factsheets

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Currently viewing:

Administrative data

Link to relevant study record(s)

Description of key information

Assessed toxicity in range of 10 - 100 mg/l from consideration of similar salts.  

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
25 mg/L

Marine water invertebrates

Marine water invertebrates
Effect concentration:

Additional information

From assessment of similar sulphonated azo substances, there is no indication that CJ321 needs to be classified as hazardous to aquatic organisms on the basis of effects to aquatic invertebrates. Although some references, including literature, indicate a nominal figure of > 100 mg/l, the read-across candidate Reactive Black 5 recorded a, EC50of 560 mg/l. Although the side groups and types of salt could make a difference, the fact that the various substances assessed were all > 100 mg/l


Testing on other similar salts resulted in effects in the range 10 – 100 mg/l (lowest ca 25 mg/l) and are classified Aquatic Chronic 3. This may be due to the aminosulphonyl group which is not present in many of the azo dyes that showed much lower toxicity. In the absence of further data, it is recommended to classify CJ321 on this basis.


The read-across is valid and is backed up by review documents, including an assessment of azo dyes by the Danish EPA that conclude low environmental toxicity to aquatic organisms.