Registration Dossier
Registration Dossier
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 463-270-2 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2000-10-19 - 2001-01-18
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: The GLP-Study was performed according to an internationally accepted guideline.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 001
- Report date:
- 2001
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Test material form:
- other: solid
- Details on test material:
- Purity: 100%
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, adapted
- Details on inoculum:
- The study was performed with aerobic activated sludge from a wastewater treatment plant (ARA Ergolz II, Fullinsdorf, Switzerland) treating predominantly domestic wastewater. The sludge was washed once with tap water by centrifugation and the supernatant liquid phase was decanted. A homogenized aliquot of the final sludge suspension was weighed, thereafter dried and the ratio of wet to dry weight was calculated.
Based on this ratio, calculated amounts of wet sludge were suspended in test water to obtain a concentration equivalent to 4 g (±10%) dry material per liter. During holding, the sludge was aerated at room temperature until use. Prior to use, the sludge was diluted with test water to a concentration of 1 g per liter (dry weight basis). This diluted activated sludge was used as inoculum to give a final concentration of 30 mg dry material per liter. - Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- 100 mg/L
- Based on:
- test mat.
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- The test water was prepared according to the testing guidelines. Analytical grade salts were dissolved in purified water to obtain the following stock solutions:
a) KH2P04 8.50 g/l K2HP04 21.75 g/l Na2HP04 x 2H20 33.40 g/l NH4CI 0.50 g/l
The pH of this solution was 7.4
b) MgS04 x 7H20 22.50 g/l
c) CaCI2 x 2H20 36.40 g/l
d) FeCI3x6H20 0.25 g/l
In order to avoid having to prepare solution d) immediately before use, one drop of concentrated HCI per liter was added.
To obtain the final test water, 10 ml of stock solution a) and 1 ml each of stock solutions b) - d) were combined and made up to 1000 ml with purified water. The pH was adjusted to 7.4 with a diluted hydrochloric acid solution.
Test Concentrations
The amounts of test item were first weighed onto slides which were then transferred into the test flasks. The reference item was added via a concentrated stock solution. No emulsifiers or solvents were used. For the test item, ultrasound dispersion was employed for fifteen minutes to obtain a more or less homogeneous suspension. To each flask (with the exception of the abiotic control) activated sludge was added. Finally, the flasks were made up to a volume of 250 ml with test water.
Reference substance
- Reference substance:
- benzoic acid, sodium salt
Results and discussion
% Degradation
- Parameter:
- % degradation (O2 consumption)
- Value:
- 35
- Sampling time:
- 28 d
Any other information on results incl. tables
Time |
Percentage Biodegradation |
||||
(days) |
Test item |
Procedure control |
Toxicity |
||
|
|
|
|
|
control |
|
Flask No. |
Flask No. |
Flask No. |
Flask No. |
Flask No. |
|
9 |
11 |
1 |
2 |
12 |
0 |
0 |
0 |
0 |
0 |
0 |
1 |
0 |
0 |
9 |
6 |
2 |
2 |
0 |
0 |
52 |
46 |
16 |
3 |
- |
— |
— |
— |
- |
4 5 |
7 |
1 |
72 |
67 |
24 |
6 |
8 |
4 |
76 |
70 |
26 |
7 |
9 |
6 |
79 |
73 |
27 |
8 |
11 |
7 |
79 |
75 |
28 |
9 |
13 |
8 |
81 |
77 |
30 |
10 |
— |
— |
— |
— |
— |
11 |
19 |
14 |
83 |
81 |
34 |
13 |
21 |
17 |
83 |
81 |
35 |
14 |
23 |
18 |
84 |
82 |
36 |
15 |
25 |
19 |
84 |
82 |
37 |
16 |
27 |
21 |
84 |
83 |
38 |
17 18 |
— |
— |
— |
— |
— |
19 |
30 |
25 |
86 |
84 |
42 |
20 |
31 |
26 |
86 |
84 |
42 |
21 |
32 |
27 |
86 |
85 |
43 |
22 |
33 |
28 |
86 |
85 |
44 |
23 |
34 |
29 |
87 |
85 |
45 |
24 |
— |
— |
— |
— |
— |
25 |
— |
— |
— |
— |
— |
26 |
36 |
31 |
87 |
86 |
46 |
27 |
37 |
32 |
87 |
87 |
46 |
28 |
38 |
33 |
88 |
87 |
48 |
Mean (Day28) |
35 |
87 |
|
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- inherently biodegradable
- Conclusions:
- YOFCO MAS was found to be moderately biodegradable under the test conditions within 28 days. However, the pass level for ready biodegradability, i.e. a biodégradation rate of at least 60% of the ThOD in a 10-day window within the 28-day period of the test, was not reached.
- Executive summary:
The test item YOFCO MAS was investigated for its ready biodegradability in a "28-Day Manometric Respirometry Test" based onEUCommission Directive 92/69 EEC, C.4-D, 1992 and OECD Guideline for Testing of Chemicals No. 301 F, 1992.
The percentbiodégradationof the test item was calculated based on the theoretical oxygen demand of 3.13 mg 02/mg test item. The theoretical oxygen demand was additionally confirmed by determination of its chemical oxygen demand which was found to be identical to the theoretical oxygen demand.
The biochemical oxygen demand (BOD) of YOFCO MAS in the test media slowly but steadily increased from Day 1 until test termination on Day 28. At the end of the exposure period, the meanbiodégradationrate of YOFCO MAS amounted to 35%. Consequently, YOFCO MAS was found to be moderately biodegradable under the test conditions within 28 days. However, the pass level for ready biodegradability, i.e.a biodégradationrate of at least 60% of the ThOD in a 10-day window within the 28-day period of the test, was not reached.
In the toxicity control, containing both YOFCO MAS and the reference item sodium benzoate, no inhibitory effect on thebiodégradationof the reference item was determined. Thus, YOFCO MAS had obviously no inhibitory effect on the activity of activated sludge microorganisms.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.

Welcome to the ECHA website. This site is not fully supported in Internet Explorer 7 (and earlier versions). Please upgrade your Internet Explorer to a newer version.
This website uses cookies to ensure you get the best experience on our websites.
Find out more on how we use cookies.