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EC number: - | CAS number: -
The average cumulative number of young per female in the control after 21 days was 120 ± 13. The reproduction curve recorded at 0.93, 1.8 and 2.7 mgtl closely followed or was even slightly above the curve of the control. The curves recorded at the higher concentrations clearly stayed behind from the beginning of reproduction. Since these higher concentrations of 4.9 and 8.3 mg/L showed a high mortality number of parental organisms no final judgement regarding reproduction was possible. There were no recordings of unhatched (aborted) eggs at any of the test concentrations throughout the 21-day test period. Immobile young were only recorded at 4.9 mg/L.
Number of living juveniles per parent animal at each nominal test concentration, mean number of neonates per concentration and coefficient of variance.
M=Mortality parent animal
The purpose of this study was to assess the toxicity of the test substance dissolved in fresh water, on the reproductive efficacy of Daphnia magna STRAUS - clone 5, in a semi-static test complying with the OECD Guideline No. 211 (OECD, 2012).
The primary test criterion used to indicate the toxicity of the test substance was reproductive capacity expressed as the total number of neonates per surviving parent animal at the end of the study due to there being no test substance related parent mortality. Data on parental length and dry-weight was also generated for use in determining secondary endpoints as required.
The nominal concentrations used in the study were as follows:
0.48, 1.53, 4.88, 15.3 and 50 mg/L including a test medium only control.
All concentrations given refer to the technical product as supplied by the sponsor.
The following quality criteria were met:
• Cultures were in good health (i.e. disease free, no ephippia or males, no discolored animals valid reference test).
• One parent animal died in the control group over the test period, which is not more than 20%.
• The average number of juveniles per parent animal alive at the end of the test in the control was 168 after 21 days (minimum acceptable = 60).
• Analytical quality criteria considered needed for a robust analysis were met.
Reproduction (Primary endpoint)
A NOEC and LOEC for reproduction for the substance as nominal test material were determined as 4.9 and 15.6 mg/L respectively. The EC10for reproduction was calculated as 4.1 mg/L. (Nominal concentrations) The NOEC for reproduction can also be expressed as 1.6 mg/L (Time weighted measured mean).
The test material may be concluded not to be toxic to the reproduction of D.Magna based on data from the primary endpoint and additional analysis to recover the test material.
One OECD 211 GLP test is available with an overall NOEC of 2.7 mg/L (mean measured concentration).
One OECD 211 GLP test with 3,6,9-Triethyl-3,6,9-trimethyl-1,4,7-triperoxonane (CAS 24748 -23 -0) is available with an overall NOEC of 2.7 mg/L (mean measured concentration).
This result is supported by an OECD 211 study according to protocol but without GLP. The NOEC for reproduction of this study is 1.6 mg/L (measured TWA concentration).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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