3-methyl-1-(2,6,6-trimethylcyclohex-1-en-1-yl)penta-1,4-dien-3-ol

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Test protocol equivalent to guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1988

Report date:

1988

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

Deviations:

no

GLP compliance:

no

Test type:

standard acute method

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

3-methyl-1-(2,6,6-trimethylcyclohex-1-en-1-yl)penta-1,4-dien-3-ol

EC Number:

226-006-5

EC Name:

3-methyl-1-(2,6,6-trimethylcyclohex-1-en-1-yl)penta-1,4-dien-3-ol

Cas Number:

5208-93-5

Molecular formula:

C15H24O

IUPAC Name:

(4E)-3-methyl-5-(2,6,6-trimethylcyclohex-1-en-1-yl)penta-1,4-dien-3-ol

Details on test material:

- Name of test material (as cited in study report): Vinyljonol

Test animals

Species:

rat

Strain:

Wistar

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Dr. Thomae
- Weight at study initiation: mean males: 181 g; mean females: 171 g
- Fasting period before study: 16 hrs
- Housing: 5 animals per cage
- Diet: ad libitum (Kliba 343)
- Water: tap water ad libitum
- Acclimation period: at least one week


ENVIRONMENTAL CONDITIONS
- Temperature (°C): 20-24
- Humidity (%): 30-70
- Photoperiod (hrs dark / hrs light): 12/12

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

olive oil

Details on oral exposure:

VEHICLE
- Justification for choice of vehicle: test substance is insoluble in water
- Concentration in vehicle: 40 g test substance/100ml

MAXIMUM DOSE VOLUME APPLIED: 5 ml/kg bw

Doses:

2000 mg/kg bw

No. of animals per sex per dose:

5

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: recording of signs and symptoms several times on the day of administration, at least once each workday; check for moribund and dead animals twice each workday and once on holidays; weighing at the beginning of the experiment and on the days 7 and 13
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight, gross pathology

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 2 000 mg/kg bw

Mortality:

- no mortalities observed

Clinical signs:

other: -males: dyspnea (4 hrs- day 1), apathy (4 hrs- day 1), staggering (4 hrs- day 1), urine orange (day 2-3), piloerection (4hrs-day 1), poor general state (4 hrs- day 1) - females: dyspnea (4 hrs- day 1), apathy (4 hrs- day 1), abnormal position (day 1), sta

Gross pathology:

- no pathological findings noted