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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1997-10-17 till 1997-10-23
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions
Remarks:
e.g. no annalytical monitoring.
Qualifier:
equivalent or similar to guideline
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
Deviations:
no
GLP compliance:
yes
Analytical monitoring:
no
Details on sampling:
Concentration: 100 mg/L nominal
Vehicle:
no
Details on test solutions:
- direct weighing
- 0.0994 g of test item in 1 litre of dilution water
- The solution wastreated of 60 seconds at 8000 rpm with an ultra turrax
- Afterwards it was measured for 3 hours on a magnetic stirrer
- The pH value was measured to be 8.0
Test organisms (species):
Daphnia magna
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h
Test temperature:
temperature range: 19.5 - 19.9
pH:
7.6
Dissolved oxygen:
8.5 - 9.1 mg/L O2
Nominal and measured concentrations:
- nominal: control and 100 mg/L
- pretested measurement: control and 1, 3.2, 10, 31.6 and 100 mg/L
- measured: control and 10, 20, 40, 50 and 80 mg/L
Reference substance (positive control):
no
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
20 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
80 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Key result
Duration:
48 h
Dose descriptor:
EC50
Remarks:
Calculated by geometric mean of EC0 and EC100
Effect conc.:
40 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility

Geometric mean: EC 0 / EC 100 : 40 mg/L

Validity criteria fulfilled:
not specified
Conclusions:
The acute toxicity of cyclohex-3-ene-1-carbaldehyde to aquatic invertebrates (Daphnia magna) was tested equivalent or similar to EU method C.2 'acute toxicity for Daphnia'. During 48 hours exposure, an EC50 of 40 mg/L was obtained.
Executive summary:

The acute toxicity of cyclohex-3-ene-1-carbaldehyde to aquatic invertebrates (Daphnia magna) was tested equivalent or similar to EU method C.2 'acute toxicity for Daphnia'. During 48 hours exposure, an EC50 of 40 mg/L was obtained.

Description of key information

The acute toxicity of cyclohex-3-ene-1-carbaldehyde to aquatic invertebrates (Daphnia magna) was tested equivalent or similar to EU method C.2 'acute toxicity for Daphnia'. During 48 hours exposure, an EC50 of 40 mg/L was obtained.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Dose descriptor:
EC50
Effect concentration:
40 mg/L

Additional information