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Toxicological information

Acute Toxicity: inhalation

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Administrative data

Endpoint:
acute toxicity: inhalation
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: acceptable well documented study report which meets basic scientific principles

Data source

Reference
Reference Type:
other: microfiche
Title:
Unnamed
Year:
1991
Report date:
1991

Materials and methods

Principles of method if other than guideline:
other: acute inhalation study
GLP compliance:
not specified
Test type:
other: acute inhalation study

Test material

Constituent 1
Chemical structure
Reference substance name:
Cyclohex-3-ene-1-carbaldehyde
EC Number:
202-858-3
EC Name:
Cyclohex-3-ene-1-carbaldehyde
Cas Number:
100-50-5
Molecular formula:
C7H10O
IUPAC Name:
cyclohex-3-ene-1-carbaldehyde
Details on test material:
1,2,3,6-tetrahydrobenzaldehyde (THBA), purity:>99%

Test animals

Species:
rat
Strain:
Sprague-Dawley
Sex:
male/female

Administration / exposure

Route of administration:
inhalation: vapour
Type of inhalation exposure:
whole body
Vehicle:
air
Analytical verification of test atmosphere concentrations:
yes
Remarks:
gas chromatograph
Duration of exposure:
4 - 8 h
Remarks on duration:
four experiments: time points: 4h, 6h and 8 h
Concentrations:
experiment I: 6 h exposure: 1679 ppm, experiment II: 8 h exposure: 1437 ppm, experiment III: 6 h exposure: 1939 ppm, experiment IV: 4h exposure: 1571 ppm
No. of animals per sex per dose:
5 per sex per dose
Control animals:
no

Results and discussion

Effect levelsopen allclose all
Sex:
male/female
Dose descriptor:
other: LT50 value
Effect level:
5.66 other: hours
Based on:
test mat.
Remarks on result:
other: LT50 value: static group 8 and 4 hours groups
Sex:
male/female
Dose descriptor:
other: dynamic exposure group
Effect level:
1 679 ppm
Based on:
test mat.
Exp. duration:
6 h
Remarks on result:
other: clinical signs
Sex:
male/female
Dose descriptor:
other: static condtions
Effect level:
1 571 ppm
Based on:
test mat.
Exp. duration:
4 h
Remarks on result:
other: clinical signs
Sex:
male/female
Dose descriptor:
other: static group
Effect level:
1 939 ppm
Based on:
test mat.
Exp. duration:
6 h
Remarks on result:
other: clinical signs, macroscopic lesions, death 10/10
Sex:
male/female
Dose descriptor:
other: static group
Effect level:
1 437
Based on:
test mat.
Exp. duration:
8 h
Remarks on result:
other: clinical signs, macroscopic lesions, death 10/10

Any other information on results incl. tables

Dynamic exposure group(6 h, 1679 ppm):

Clinical signs: day of exposure: blepharospasm, lacrimation, severe periocular encrustation, perinasal wetness/encrustation, hypoactivity, mouth breathing and audible respiration

postexposure period: periocular encrustation, unkempt fur, and hypoactivity on the day following exposure

Mortaility: no rat died during exposure or during the 14 -day postexposure period

Body weights: mean body weight gain was observed for both sexes at 7 and 14 days following the day of exposure

Necropsy: No macroscopic lesions were observed in animals sacrificed at the end of the 2 -week post-exposure period

Static exposure (4h 1571 ppm, 6 h 1939 ppm, 8 h 1437 ppm):

Clinical signs: all static groups during exposure: blepharospasm, lacrimation, perinasal/perioral wetness, abdominal and/or mouth breathing hypoactivity, and an absence of startle reflex and motor activity.

Clinical signs observed on the day following exposure (6 and 8 h groups): lacrimation, narcosis and /or prostation, decreased respiratory rate, and an absence of toe and tail pinch, eye blink, and surface righting reflexes

Clinical signs observed during the subsequent postexposure period (6h and 8 h groups): periocular/perinasal encrustation, urogenital area wetness, hypoactivity, and decreased respiration rate

Clinical signs (4 h group postexposure): Narcosis was observed for the first 5 minutes following the end of exposure, followed by ataxia and periocular/perinasal wetness

Mortality: 4h group: 0/10, 6 h group 10/10, 8 h group 10/10, death occurred 1 to 4 days after exposure

LT50 value calculated: 5.66 (4.19 to 7.64)

Body weight gain: 4 h group: body weight gain observed 7 and 14 days after exposure

Necropsy of decedent (6 h and 8 h group: mottled red or bright pink discoloration of lungs, yellow staining of the urogential area, multiple ulcers on the tail, feet and paws, red or dark red discoloration of the thymus, liver, kidneys and brain, hemorrhages into the meninges or posterior cranial fossa (two animals 6 h group)

Applicant's summary and conclusion

Executive summary:

Acute inhalation study