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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Toxicity to aquatic plants other than algae

Administrative data

Endpoint:
toxicity to aquatic plants other than algae
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Published in peer reviewed literature, minor restrictions in design and/or reporting but otherwise adequate for assessment

Data source

Reference
Reference Type:
publication
Title:
Comparative Chronic Toxicity of Pyridine, α-Picoline, and β-Picoline to Lemna minor L. and Chlorella vulgaris B
Author:
Singh and Chandra
Year:
2005
Bibliographic source:
Bull. Environ. Contam. Toxicol. (2005) 75:482–489

Materials and methods

Test guideline
Qualifier:
no guideline followed
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
2-methylpyridine
EC Number:
203-643-7
EC Name:
2-methylpyridine
Cas Number:
109-06-8
Molecular formula:
C6H7N
IUPAC Name:
2-methylpyridine
Details on test material:
- Name of test material (as cited in study report): α-picoline

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION
120 x 15 mm-petri-dish with 100 mL in double strength algal growth medium without EDTA containing different concentrations test substance.

Test organisms

Test organisms (species):
Lemna minor
Details on test organisms:
TEST ORGANISM
- Common name: Duckweed
- Strain: Lemna minor L.
- Source: Collected form pollution free sites. Water samples were taken to confirm that the water bodies were not loden with pollutants.
- Method of cultivation: The duckweed test specimens were selected from the axenic stock culture 24 h before the actual test. Selection criteria were healthy-looking and two fronds of approximately equal size per colony.

ACCLIMATION
- Acclimation period: 4 weeks
- Culture media: double strenght algal growth medium without EDTA

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
14 d

Test conditions

Hardness:
245 ± 14 mg/L
Test temperature:
25 ± 2 °C
pH:
8.1 ± 0.2
Nominal and measured concentrations:
- Nominal concentrations (v/v): 0.00125, 0.0025, 0.005, 0.0125, 0.025, 0.0375, and 0.05%
- Nominal concentrations mg/L: approx. 11.75, 23.5, 47, 117.5, 235, 352.5, and 470 (calculated with the density 0.94 g/cm3)
- Analytical concentrations: not determined
Details on test conditions:
TEST SYSTEM
- Test vessel: 120 x 15 mm-petri-dish
- No. of organisms per vessel: 50 colonies
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 3
- Shaking rate: Twice daily by hand

GROWTH MEDIUM
- double strength algal growth medium

OTHER TEST CONDITIONS
- Photoperiod: 12hr light/12 hr dark
- Light intensity and quality: Cool, white and fluorescent. 115 µmol photon / m-2·s-1

EFFECT PARAMETERS MEASURED
- Determination of cell concentrations: Protein content was determined in accordance with method described by Bradford (1976)
- Chlorophyll measurement: Total chlorophyll content was determined by acetone (80%) extraction.
- Biomass: determined by the dry weight method.


Results and discussion

Effect concentrationsopen allclose all
Duration:
14 d
Dose descriptor:
IC50
Effect conc.:
ca. 836.6 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
biomass
Duration:
14 d
Dose descriptor:
IC50
Effect conc.:
ca. 780.2 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: Chlorophyll
Duration:
14 d
Dose descriptor:
IC50
Effect conc.:
ca. 554.6 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: protein
Reported statistics and error estimates:
Data were statistically analysed by an overall one-way analysis of variance (ANOVA) and when differences observed were significant, the means were compared by the Tukey-Kramer Multiple Ccomparison Test. IC50 values were graphically estimated.

Applicant's summary and conclusion