2-methylpyridine

Toxicity to microorganisms

Administrative data

Endpoint:

toxicity to microorganisms

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: GLP status not known, near guideline study, available as unpublished report, minor restrictions in design and reporting but otherwise adequate for assessment

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1990

Report date:

1990

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

DIN 38412-8 (Pseudomonas Zellvermehrungshemmtest)

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

2-methylpyridine

EC Number:

203-643-7

EC Name:

2-methylpyridine

Cas Number:

109-06-8

Molecular formula:

C6H7N

IUPAC Name:

2-methylpyridine

Details on test material:

- Name of test material (as cited in study report): 2-Methylpyridin

Sampling and analysis

Analytical monitoring:

no

Test solutions

Details on test solutions:

Concentration stock solution: 12500 mg/L

Test organisms

Test organisms (species):

Pseudomonas putida

Details on inoculum:

- Strain: dsm 50026
- Medium: AK-Medium according to DIN 38412 part 8
- Temperature: ca. 24°C
- Culture vessel: 300 mL erlenmeyer filled up to 100 mL
- Culture shaken: yes, at 150 movements per minute
- Culture duration: 7h ± 1h

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

16 h

Remarks on exposure duration:

± 1 hour

Test conditions

Test temperature:

ca. 20 °C

Nominal and measured concentrations:

- Nominal concentrations: 0 (control), 39.09, 78.13, 156.25, 312.5, 625, 1250, 2500, 5000, and 10000 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: 10 mL
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Biomass loading rate: 1 mL of a Pseudomonas suspension adjusted to a turbidity of 10 TE/F

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: AK medium according to DIN 38412, part 8 (draft)

EFFECT PARAMETERS MEASURED:
Photometric determination of light extinction after 16 hours of exposure. measurements were performed at 436 nm.

Reference substance (positive control):

not specified

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

16 h

Dose descriptor:

EC50

Effect conc.:

2 106.9 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth inhibition

Effect concentrations 2

Duration:

16 h

Dose descriptor:

EC10

Effect conc.:

1 403.6 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth inhibition

Details on results:

See: "Any other information on results incl. tables"

Any other information on results incl. tables

Growth inhbition:

Test conc. (mg/L)	% difference to control
0 (control)	-
39.09	107.21
78.13	106.77
156.25	106.55
312.5	105.98
625	104.15
1250	98.73
2500	27.64
5000	0.22
10000	0.35