Registration Dossier

Administrative data

Endpoint:
toxicity to microorganisms
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: GLP status not known, near guideline study, available as unpublished report, minor restrictions in design and reporting but otherwise adequate for assessment

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1990
Report date:
1990

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
DIN 38412-8 (Pseudomonas Zellvermehrungshemmtest)
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
2-methylpyridine
EC Number:
203-643-7
EC Name:
2-methylpyridine
Cas Number:
109-06-8
Molecular formula:
C6H7N
IUPAC Name:
2-methylpyridine
Details on test material:
- Name of test material (as cited in study report): 2-Methylpyridin

Sampling and analysis

Analytical monitoring:
no

Test solutions

Details on test solutions:
Concentration stock solution: 12500 mg/L

Test organisms

Test organisms (species):
Pseudomonas putida
Details on inoculum:
- Strain: dsm 50026
- Medium: AK-Medium according to DIN 38412 part 8
- Temperature: ca. 24°C
- Culture vessel: 300 mL erlenmeyer filled up to 100 mL
- Culture shaken: yes, at 150 movements per minute
- Culture duration: 7h ± 1h

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
16 h
Remarks on exposure duration:
± 1 hour

Test conditions

Test temperature:
ca. 20 °C
Nominal and measured concentrations:
- Nominal concentrations: 0 (control), 39.09, 78.13, 156.25, 312.5, 625, 1250, 2500, 5000, and 10000 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: 10 mL
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Biomass loading rate: 1 mL of a Pseudomonas suspension adjusted to a turbidity of 10 TE/F

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: AK medium according to DIN 38412, part 8 (draft)

EFFECT PARAMETERS MEASURED:
Photometric determination of light extinction after 16 hours of exposure. measurements were performed at 436 nm.
Reference substance (positive control):
not specified

Results and discussion

Effect concentrationsopen allclose all
Duration:
16 h
Dose descriptor:
EC50
Effect conc.:
2 106.9 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth inhibition
Duration:
16 h
Dose descriptor:
EC10
Effect conc.:
1 403.6 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth inhibition
Details on results:
See: "Any other information on results incl. tables"

Any other information on results incl. tables

Growth inhbition:

Test conc. (mg/L)

% difference to control

0 (control)

-

39.09

107.21

78.13

106.77

156.25

106.55

312.5

105.98

625

104.15

1250

98.73

2500

27.64

5000

0.22

10000

0.35

Applicant's summary and conclusion