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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Specific investigations: other studies

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Administrative data

biochemical or cellular interactions
Type of information:
experimental study
Adequacy of study:
supporting study
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Non guideline study. GLP-status unknown. Published in peer reviewed literature. Restrictions in design and reporting but adequate for assessment.

Data source

Reference Type:
Pyridine and imidazole reversibly inhibit the respiratory burst in porcine and human neutrophils: Evidence for the involvement of cytochrome b558 in the reaction
Iizuka T., Kanegasaki S., Makino R., Tanaka T., Ishimura Y.
Bibliographic source:
Biochemical And Biophysical Research Communications, Vol 130(2), 621-626

Materials and methods

Principles of method if other than guideline:
Porcine and human neutrophils were isolated from peripheral blood. Oxygen consumption was measured spectrophotometrically after addition of the test substance.
GLP compliance:
not specified
Type of method:
in vitro
Endpoint addressed:
other: Respiratory burst

Test material

Constituent 1
Chemical structure
Reference substance name:
EC Number:
EC Name:
Cas Number:
Molecular formula:
Details on test material:
- Name of test material (as cited in study report): 2-methylpyridine

Test animals

other: Porcine and human neutrophils

Administration / exposure

Details on study design:
Porcine and human neutrophils were isolated from peripheral blood. Oxygen consumption was measured. The cells in Hanks' solution were stimulated by 100 ng/mL of PMA or 2.5 mg/mL of normal-serum opsonized zymosan. Absorption spectrophotometry at 77 K was carried out with spectrophotometer equipped with a low temperature attachment.

Results and discussion

Details on results:
The test substance inhibited the respiratory burst in neutrophils. Inhibition was accompanied by a spectral change in reduced cytochrome B558. Both changes were reversible.
The test substance inhibited the NADPH-dependent oxygen consumption in PMA-stimulated lysed cells. Oxygen consumption in these circumstances was restored by the addition of NADPH.

Applicant's summary and conclusion