2-methylpyridine

Dermal absorption

Administrative data

Endpoint:

dermal absorption in vitro / ex vivo

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Non guideline study. GLP-status unknown. Published abstract in peer reviewed literature.

Data source

Reference

Reference Type:

publication

Title:

Dermal Absorption of Pyridine and Some Methyl Pyridines In Vitro

Author:

Wilkinson, S.C., Williams, F.M.

Year:

2001

Bibliographic source:

Toxicology 168 (2001) 1

Materials and methods

Principles of method if other than guideline:

The absorption of the test substance through human skin was studied in vitro using dermatomed human breast skin (280 µm) to assess the dermal absorption hazard of this compound and to identify physicochemical influences on absorption

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

2-methylpyridine

EC Number:

203-643-7

EC Name:

2-methylpyridine

Cas Number:

109-06-8

Molecular formula:

C6H7N

IUPAC Name:

2-methylpyridine

Details on test material:

- Name of test material (as cited in study report): 2-methyl pyridine (MPYR)

Test animals

Species:

other: Dermatomed human breast skin (280 µm)

Administration / exposure

Details on in vitro test system (if applicable):

- Dermatomed human breast skin (280 µm) was mounted in Scott Dick flow through diffusion cells.
- Minimal Essential Medium Eagle (pH 7.4) was pumped beneath the skin at 1.5 mL/h and at 32 °C for 24 h.
- The test compound was applied to the skin (0.64 cm2) undiluted (10 µL) or in aqueous vehicle (200 µL, 3 mg/mL).
- Charcoal filters were placed above the skin to trap volatilized test compounds.
- Concentrations of test compounds in receptor fluids were determined by Gas Chromatography.
- Steady-state flux (J), apparent kp, time to steady state and total absorption (% of applied dose) were calculated from cumulative absorption time curves.

Results and discussion

Any other information on results incl. tables

Dermal absorption parameters for 2-methylpyridine

Undiluted:

- Steady-state flux = 822 ± 222 nmol/cm2 per h, apparent kp = 0.8 ± 0.2E−4 cm/h, Time to steady-state = 0.55 ± 0.15 h

- Total absorption = 0.49 ± 0.14 %

Aqueous solution:

- Steady-state flux = 686 ± 129 nmol/cm2 per h, apparent kp = 213.0 ± 40.0E−4 cm/h, Time to steady-state = 0.39 ± 0.08 h

- Total absorption = 5.56 ± 1.18 %