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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

dermal absorption in vitro / ex vivo
Type of information:
experimental study
Adequacy of study:
supporting study
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Non guideline study. GLP-status unknown. Published abstract in peer reviewed literature.

Data source

Reference Type:
Dermal Absorption of Pyridine and Some Methyl Pyridines In Vitro
Wilkinson, S.C., Williams, F.M.
Bibliographic source:
Toxicology 168 (2001) 1

Materials and methods

Principles of method if other than guideline:
The absorption of the test substance through human skin was studied in vitro using dermatomed human breast skin (280 µm) to assess the dermal absorption hazard of this compound and to identify physicochemical influences on absorption
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
EC Number:
EC Name:
Cas Number:
Molecular formula:
Details on test material:
- Name of test material (as cited in study report): 2-methyl pyridine (MPYR)

Test animals

other: Dermatomed human breast skin (280 µm)

Administration / exposure

Details on in vitro test system (if applicable):
- Dermatomed human breast skin (280 µm) was mounted in Scott Dick flow through diffusion cells.
- Minimal Essential Medium Eagle (pH 7.4) was pumped beneath the skin at 1.5 mL/h and at 32 °C for 24 h.
- The test compound was applied to the skin (0.64 cm2) undiluted (10 µL) or in aqueous vehicle (200 µL, 3 mg/mL).
- Charcoal filters were placed above the skin to trap volatilized test compounds.
- Concentrations of test compounds in receptor fluids were determined by Gas Chromatography.
- Steady-state flux (J), apparent kp, time to steady state and total absorption (% of applied dose) were calculated from cumulative absorption time curves.

Results and discussion

Any other information on results incl. tables

Dermal absorption parameters for 2-methylpyridine


- Steady-state flux = 822 ± 222 nmol/cm2 per h, apparent kp = 0.8 ± 0.2E−4 cm/h, Time to steady-state = 0.55 ± 0.15 h

- Total absorption = 0.49 ± 0.14 %

Aqueous solution:

- Steady-state flux = 686 ± 129 nmol/cm2 per h, apparent kp = 213.0 ± 40.0E−4 cm/h, Time to steady-state = 0.39 ± 0.08 h

- Total absorption = 5.56 ± 1.18 %

Applicant's summary and conclusion