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Toxicological information

Acute Toxicity: inhalation

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Administrative data

Endpoint:
acute toxicity: inhalation
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Non GLP, non guideline study. Published in peer reviewed literature. Minor restrictions in design and reporting but adequate for assessment.

Data source

Reference
Reference Type:
publication
Title:
Comparative acute toxicity and primary irritancy of various classes of amines
Author:
Myers, R.C., and Ballantyne, B.
Year:
1997
Bibliographic source:
Toxic Substance Mechanisms 16, 151-193

Materials and methods

Principles of method if other than guideline:
A constant exposure time of 4 hours was used and the concentration of test material in the chamber air was varied until mortality data were sufficient to allow the calculation of an LC50. Rats were examined frequently during exposure and daily for 14 post-exposure days. All rats that died and sacrificed survivors were subjected to necropsy.
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
2-methylpyridine
EC Number:
203-643-7
EC Name:
2-methylpyridine
Cas Number:
109-06-8
Molecular formula:
C6H7N
IUPAC Name:
2-methylpyridine
Details on test material:
- Name of test material (as cited in study report): a-Picoline (2-methyl-pyridine)
- Physical state: Liquid

Test animals

Species:
rat
Strain:
Wistar
Sex:
female
Details on test animals or test system and environmental conditions:
TEST ANIMALS
- Age at study initiation: 3 - 4 wk
- Weight at study initiation: 90 - 120 g
- Fasting period before study: No fasting took place
- Diet: Commercial rodent feed ad libitum
- Water: Municipal water, ad libitum

Administration / exposure

Route of administration:
inhalation: vapour
Type of inhalation exposure:
whole body
Vehicle:
other: dried air
Details on inhalation exposure:
- The test material was pumped into the top of a vertical glass evaporator tube, which was heated sufficiently to cause vaporization (Snelling & Dodd, 1990). Dried air was introduced through the bottom of the tube (counter-current to the sample flow) and the resultant vapor-containing atmosphere passed to a 9-L exposure chamber.
- Chamber concentrations were determined by adjustment of sample and/or air flow rates.
Analytical verification of test atmosphere concentrations:
not specified
Duration of exposure:
4 h
No. of animals per sex per dose:
6
Control animals:
no
Details on study design:
- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: Body weights were measured just before exposure and at sacrifice.
- Necropsy of survivors performed: yes
- Other examinations performed: histopathology
Statistics:
LC50 were calculated along with 95% confidence limits using the method of moving averages (Thompson, 1947; Weil, 1983)

Results and discussion

Effect levels
Sex:
female
Dose descriptor:
LC50
Effect level:
2 100 ppm
95% CL:
1 700 - 2 600
Exp. duration:
4 h
Remarks on result:
other: 7993.9 mg/m3
Mortality:
Times to death: 1.7 hour - 1 day

Applicant's summary and conclusion

Interpretation of results:
toxic
Remarks:
Migrated information