2-methylpyridine

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Non GLP, non guideline study. Published in peer reviewed literature. Minor restrictions in design and reporting but adequate for assessment.

Data source

Reference

Reference Type:

publication

Title:

Comparative acute toxicity and primary irritancy of various classes of amines

Author:

Myers, R.C., and Ballantyne, B.

Year:

1997

Bibliographic source:

Toxic Substance Mechanisms 16, 151-193

Materials and methods

Principles of method if other than guideline:

A constant exposure time of 4 hours was used and the concentration of test material in the chamber air was varied until mortality data were sufficient to allow the calculation of an LC50. Rats were examined frequently during exposure and daily for 14 post-exposure days. All rats that died and sacrificed survivors were subjected to necropsy.

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

2-methylpyridine

EC Number:

203-643-7

EC Name:

2-methylpyridine

Cas Number:

109-06-8

Molecular formula:

C6H7N

IUPAC Name:

2-methylpyridine

Details on test material:

- Name of test material (as cited in study report): a-Picoline (2-methyl-pyridine)
- Physical state: Liquid

Test animals

Species:

rat

Strain:

Wistar

Sex:

female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Age at study initiation: 3 - 4 wk
- Weight at study initiation: 90 - 120 g
- Fasting period before study: No fasting took place
- Diet: Commercial rodent feed ad libitum
- Water: Municipal water, ad libitum

Administration / exposure

Route of administration:

inhalation: vapour

Type of inhalation exposure:

whole body

Vehicle:

other: dried air

Details on inhalation exposure:

- The test material was pumped into the top of a vertical glass evaporator tube, which was heated sufficiently to cause vaporization (Snelling & Dodd, 1990). Dried air was introduced through the bottom of the tube (counter-current to the sample flow) and the resultant vapor-containing atmosphere passed to a 9-L exposure chamber.
- Chamber concentrations were determined by adjustment of sample and/or air flow rates.

Analytical verification of test atmosphere concentrations:

not specified

Duration of exposure:

4 h

No. of animals per sex per dose:

6

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: Body weights were measured just before exposure and at sacrifice.
- Necropsy of survivors performed: yes
- Other examinations performed: histopathology

Statistics:

LC50 were calculated along with 95% confidence limits using the method of moving averages (Thompson, 1947; Weil, 1983)

Results and discussion

Effect levels

Sex:

female

Dose descriptor:

LC50

Effect level:

2 100 ppm

95% CL:

1 700 - 2 600

Exp. duration:

4 h

Remarks on result:

other: 7993.9 mg/m3

Mortality:

Times to death: 1.7 hour - 1 day

Applicant's summary and conclusion

Interpretation of results:

toxic

Remarks:

Migrated information