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Toxicological information

Skin irritation / corrosion

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Administrative data

Endpoint:
skin irritation: in vivo
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Non GLP, non guideline study. Available as unpublished report. Restrictions in design and reporting but adequate for assessment.

Data source

Referenceopen allclose all

Reference Type:
study report
Title:
Unnamed
Year:
1983
Report date:
1983
Reference Type:
review article or handbook
Title:
SCREENING-LEVEL HAZARD CHARACTERIZATION Pyridine and Pyridine Derivatives Category
Author:
U.S. Environmental Protection Agency
Year:
2009
Bibliographic source:
http://www.epa.gov/hpvis/hazchar/Category%20Pyr%20and%20Pyr%20Derivs_Sept2009.pdf

Materials and methods

Principles of method if other than guideline:
0.5 mL of the test substance was applied to the skin of New Zealand White rabbits and covered with a patch for 4 hours. After exposure, the site was washed with water. Observations were made at removal of the test patch and at 24 and 48 hours.
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
2-methylpyridine
EC Number:
203-643-7
EC Name:
2-methylpyridine
Cas Number:
109-06-8
Molecular formula:
C6H7N
IUPAC Name:
2-methylpyridine
Details on test material:
- Name of test material (as cited in study report): Alpha Picoline

Test animals

Species:
rabbit
Strain:
New Zealand White
Details on test animals or test system and environmental conditions:
TEST ANIMALS
- Housing: Stainless steel with elevated wire mesh flooring; 1 rabbit/cage
- Diet: Wayne 15% Rabbit Ration, ad libitum
- Water: Tap water, ad libitum

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 15.6 - 23.9
- Humidity (%): 40-45
- Photoperiod (hrs dark / hrs light): 12/12

Test system

Type of coverage:
occlusive
Preparation of test site:
clipped
Vehicle:
unchanged (no vehicle)
Controls:
no
Amount / concentration applied:
TEST MATERIAL
- Amount(s) applied: 0.5 mL
Duration of treatment / exposure:
4 hours
Observation period:
48 hours
Number of animals:
6
Details on study design:
TEST SITE
- Area of exposure: approximately one square inch.
- Type of wrap if used: Surgical gauze patches measuring not less than 1" x 1" and two single layers thick were placed over the treated area and impervious material was wrapped snugly around the trunk of the animal to hold the patch in place.

REMOVAL OF TEST SUBSTANCE
- Washing: yes, with water
- Time after start of exposure: 48 hours

SCORING SYSTEM:
Corrosion was considered to have resulted if the material in contact with the rabbit skin had caused destruction or irreversible alteration of the tissue. Tissue destruction was considered to have occurred if, at any of the readings, there was ulceration or necrosis. Tissue destruction was considered to have occurred if merely sloughing of the epidermis, erythema, edema or fissuring was evident. Observations of skin irritation, if any, encountered during testing of the material were noted.

Results and discussion

In vivo

Results
Remarks on result:
other: See "Irritant/corrosive response data"
Irritant / corrosive response data:
- 4 hours after exposure: Animal #1, 2, 3, 4 and 6 showed severe erythema, moderate edema and corrosion to the skin. Animal 5 showed slight erythema, moderate edema and no corrosion to the skin.
- 24 and 48 hours after exposure: Animal #1, 2 and 3 showed severe erythema, very slight edema and corrosion to the skin. Animal #4, 5 and 6 showed severe erythema, corrosion to the skin but no edema was observed.

Applicant's summary and conclusion

Interpretation of results:
corrosive
Remarks:
Migrated information