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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1997
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions
Qualifier:
according to guideline
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
Deviations:
no
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
not applicable
GLP compliance:
no
Analytical monitoring:
no
Vehicle:
no
Details on test solutions:
A solution of the test item was prepared at a concentration of 10000 mg/L. The test solution was treated for 1 hour with an ultra turrax, stirring for 24 hours and filtered (folded filters with pore size 7-12 µm) to exclude undissolved test item.
Test organisms (species):
Daphnia magna
Details on test organisms:
- Name: Daphnia magna STRAUS, parthenogenetic females
- Source: Strain of Bundesgesundheitsamt Berlin
- Test animals: Laboratory breeding of Bayer AG Leverkusen, Germany
- Age: 0-24 hours
- Holding water: M4-Medium (Elendt, vers. BGA, 1992)
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h
Hardness:
13.8 dH
Test temperature:
20.5 °C
pH:
7.4
Dissolved oxygen:
9.6 mg/L
Salinity:
n.a.
Conductivity:
no data
Nominal and measured concentrations:
10000 mg/L (nominal), direct weighing
Details on test conditions:
EXPOSURE CONDITIONS:
- Test vessels: cylindrical glass test vessel (4 x 6.5 cm) holding 10 neonates in 20 mL of test medium
- Experimental design: 1 test concentration plus 1 control; 10 neonates per vessel, 2 replicates per concentration/control
- Method of initiation: neonates were placed in prepared media
- Feeding: no feeding during the exposure period
- Aeration: none
- Medium renewal: none
- Duration of exposure: 48 hours
- Criteria of effects: The criterion of adverse effects used in this study was the item-induced alteration of the normal mobility behaviour and the loss of motory actions of the neonates, observed at 24 and 48 hours

AUXILIARIES:
- ultrasonic bath
- magnetic stirrer
- folded filter
Reference substance (positive control):
no
Duration:
24 h
Dose descriptor:
EC0
Effect conc.:
>= 10 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Key result
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
>= 10 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Validity criteria fulfilled:
yes
Remarks:
immobilisation and other abnormalities in the controls did not exceed 10% by the end of the test; dissolved oxygen concentration remained above 3 mg/L throughout the exposure period
Conclusions:
No toxic effects against daphnia were observed at a limit test concentration of 10000 mg/L (EC0 (48h) >= 10000 mg/L) under exposure conditions. The results are expressed in terms of nominal concentrations (at 24 and 48 h).
Executive summary:

The study was performed according to Method C.2 ‘Acute toxicity for Daphnia’ (1992), described in the Council Regulation (EC) No 92/69, which is equivalent to OECD Guideline for Testing of Chemicals No. 202 'Daphnia sp., Acute Immobilization Test'.  Daphnids were exposed to the test item added to water at a limit test concentration of nominally 10000 mg/L for a period of 48 hours. At this concentration no toxic effects against Daphnia were observed at the end of the 48 hour study period, thus no statistical analysis was required to determine the EC50.  Immobilization rates were recorded at 24-hour intervals. Additionally any abnormal behavior or appearance of the Daphnia was reported every 24 hours. During the test a temperature of 20.5 °C was maintained in the test vessels. Auxiliaries used to prepare the test media were an ultrasonic bath, a magnetic stirrer and a folded filter. No specific analysis was performed. No toxic effects against daphnia were observed at a limit test concentration of 10000 mg/L (EC0 (48h) >= 10000 mg/L) under exposure conditions. The results are expressed in terms of nominal concentrations (at 24 and 48 h). This toxicity study is classified as acceptable and satisfies the guideline requirements for the acute Daphnia study.

Description of key information

No toxic effects on daphnia were observed at a limit test concentration of 10000 mg/L (EC0 (48h) >= 10000 mg/L)

under exposure conditions. The results are expressed in terms of nominal concentrations.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
10 000 mg/L

Additional information

"Should read: > 10000 mg/L"