Formaldehyde, reaction products with N,N-dimethylbenzenamine and N-methylbenzenamine, oxidized, hydrochlorides

Endpoint summary

Description of key information

Short-term toxicity to fish:

Based on the QSAR prediction done using the EPI Suite ECOSAR version 1.10, the 96 hours LC50 was estimated to be 7859.329 mg/L on fish for substance Formaldehyde, reaction products with N,N-dimethylbenzenamine and N-methylbenzenamine, oxidized, hydrochlorides with mortality effects. Thus based on this value it can be concluded that the substance can be classified as non-toxic as per the criteria of CLP regulation.

Long term toxicity to fish:

Based on theECOSAR prediction toolbox used by SSS (2016), estimated long term toxicity to fish for Formaldehyde, reaction products with N,N-dimethylbenzenamineand N-methylbenzenamine, oxidized, hydrochlorides was obtained to be 452.89 mg/L.

Short term toxicity to aquatic invertebrates:

The median effective concentration (EC50) for the test substance Formaldehyde, reaction products with N,N-dimethylbenzenamine and N-methylbenzenamine, oxidized, hydrochlorides, in Daphnia magna was determined to be 0.037 mg/L on the basis of mobiity inhibition effects in a 48 hour study. This value indicates that the substance is likely to be hazardous to aquatic invertebrates and can be classified as aquatic acute/ chronic 1 category as per the CLP classification criteria.

Long term toxicity to aquatic invertebrates:

Based on theECOSAR prediction toolbox used by SSS (2016), estimated long term toxicity of Formaldehyde, reaction products with N,N-dimethylbenzenamineand N-methylbenzenamine, oxidized, hydrochloridesto daphnid was obtained to be 171.74 mg/L.

Toxicity to algae and cyanobacteria:

The median effective concentration (ErC50) for the test substance Formaldehyde, reaction products with N,N-dimethylbenzenamine and N-methylbenzenamine, oxidized, hydrochlorides, in Desmodesmus subspicatus was determined to be 0.33 mg/L. This value indicates that the substance is likely to be hazardous to aquatic algae and can be classified as aquatic acute/chronic 1 category as per the CLP criteria.

Additional information

Short-term toxicity to fish:

By applying weight of evidence approach the studies of chemical Formaldehyde, reaction products with N,N-dimethylbenzenamine and N-methylbenzenamine, oxidized, hydrochlorides (Cas no. 83968-28-9) are summarized as follows:

From QSAR prediction done using the EPI Suite ECOSAR version 1.10 for target chemical substance Formaldehyde, reaction products with N,N-dimethylbenzenamine and N-methylbenzenamine, oxidized, hydrochlorides the 96 hours LC50 was estimated to be 7859.329 mg/L on fish with mortality effects. Thus based on this value it can be concluded that the substance can be classified as non-toxic as per the criteria of CLP regulation.

Read across study from Superior, WI:Center Lake Superior Environmental Studies Univ.Wisconsin Superior, 1984 Acute Toxicities of Organic Chemicals to Fathead Minnows (Pimephales Promelas) experimental study indicate the lethal concentration (LC50) value of N,N-dimethylaniline in fish [Pimephales promelas] in a 96 hr study was 78.2 mg/L(confidence limit 74.2 - 82.4 mg/L) in fresh water and flow-through condition.

But the test substance is readily biodegradable in water and also bioaccumulation potential not ≥ 500 (i.e reported 3.2 L/kg) all these condition support to that the test substance is non toxic to aquatic environment.

Based on the above discussions from various sources concluded that in short term exposure to fish test chemical N,N-dimethylbenzenamine and N-methylbenzenamine, oxidized, hydrochlorides (Cas no. 83968-28-9) is non toxic to fish.

Long term toxicity to fish:

Based on theECOSAR prediction toolbox used by SSS (2016), estimated long term toxicity to fish for Formaldehyde, reaction products with N,N-dimethylbenzenamineand N-methylbenzenamine, oxidized, hydrochlorides was obtained to be 452.89 mg/L.

Short term toxicity to aquatic invertebrates:

Aim of the study was to determine the nature of chemical Formaldehyde, reaction products with N,N-dimethylbenzenamine and N-methylbenzenamine, oxidized, hydrochlorides on the mobility of daphnids. Test was performed according to OECD Guideline 202.

The stock solution 10 mg/l was prepared by dissolving dark violet powder in reconstituted water. Test solutions of required concentrationas were prepared by mixing the stock solution of the test sample with reconstituted test water. The test substance was tested at the concentrations 0, 0.0016, 0.0032, 0.0065, 0.0130, 0.0260, 0.0500, 0.1500, 0.3000, 1.2500, 2.5000 mg/l. Effects on immobilisation were observed for 48 hours. With the test chemical positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. Test conducted in 50 ml glass vessel filled with 25 ml of test solution and performed in 4 replicates. After the exposure of chemical for 48 hrs, EC50 was calculated using non linear regression by the software Prism 4.0.

The median effective concentration (EC50) for the test substance Formaldehyde, reaction products with N,N-dimethylbenzenamine and N-methylbenzenamine, oxidized, hydrochlorides, in Daphnia magna was determined to be 0.037 mg/L on the basis of mobiity inhibition effects in a 48 hour study. This value indicates that the substance is likely to be hazardous to aquatic invertebrates and can be classified as aquatic acute/chronic 1 category as per the CLP classification criteria.

Long term toxicity to aquatic invertebrates:

Based on theECOSAR prediction toolbox used by SSS (2016), estimated long term toxicity of Formaldehyde, reaction products with N,N-dimethylbenzenamineand N-methylbenzenamine, oxidized, hydrochloridesto daphnid was obtained to be 171.74 mg/L.

Toxicity to algae and cyanobacteria:

Freshwater algal growth inhibition test was carried out on Desmodesmus subspicatus with the substance Formaldehyde, reaction products with N,N-dimethylbenzenamine and N-methylbenzenamine, oxidized, hydrochlorides (83968 -28 -9). Test was conducted according to OECD Guideline 201.

The stock solution 10 mg/l was prepared by dissolving dark violet powder in OECD growth medium. Test solutions of required concentration were prepared by mixing the stock solution of the test sample with OECD growth medium and inoculum culture and tested at the concentrations 0, 0.13, 0.20, 0.30, 0.45, 0.68 mg/l. Effects on the growth rate of the organism were studied. Apart from test chemical, positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. Test conducted in 50 ml glass vessel filled with 7.5 ml of test solution and performed in 3 replicates. After the exposure of chemical for 72 hrs, ErC50 was calculated using non linear regression by the software Prism 4.0.

The median effective concentration (ErC50) for the test substance Formaldehyde, reaction products with N,N-dimethylbenzenamine and N-methylbenzenamine, oxidized, hydrochlorides, in Desmodesmus subspicatus was determined to be 0.33 mg/L. This value indicates that the substance is likely to be hazardous to aquatic algae and can be classified as aquatic acute/chronic 1 category as per the CLP criteria.