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Short-term toxicity to fish

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Description of key information

The short term toxicity to fish -Oryzias latipes was estimated by the QSAR Toolbox version 3.3 prediction the 96 hours LC50 was estimated to be 294.099151611 mg/l in semi static condition for test substance 4-amino-5-hydroxynaphthalene-1,7-disulphonic acid with mortality effects.
This LC50 value of test chemical indicate that it is non toxic to aquatic environment thus not consider for further classification.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Effect concentration:
294.099 mg/L

Additional information

By applying the weight of evidence approch to Short term fish toxicity study for Target -7-Amino-1-hydroxy-3-naphthalenesulfonic acid(Cas no.130-23-4)Summarized as fallowed:

Study from SSS QSAR prediction model, 2016 for target chemical 4-amino-5-hydroxynaphthalene-1,7-disulphonic acid estimates the short term toxicity to fish -Oryzias latipes at 96 hours LC50 which is to be 294.099151611 mg/l in semi static condition and with mortality effects.

Above target prediction supported by read across chemical (Cas no.87-02) from peer reviewed study(GREIM H. et al;Chemosphere; Vol. 28, No. 12, pp. 2203.2236, 1994)The investigation was performed to determine the toxicity of sulphonic acids to fish the lethal concentration for test substance 7-Amino-4 hydroxy- 2 – naphthalenesulphonic acid in short term fish toxicity (LC0) was determined to be 1000 mg/l. At this dose

concentration mortality was not observed.

This LC0 value indicate that the test substance 7-Amino-4 hydroxy- 2 – naphthalenesulphonic acid is non toxic to fish upto dose concentration 1000 mg/l.


Another study of read across substance (Cas no. 90-51-7) from Study report of Vernon C. Applegate,;Special Scientific Report- Fisheries No. 207;1957 as the short term toxicity test to rainbow trout, bluegill and sea lamprey was conducted for 24 hrs.

Test organisms Sea lamprey (Petromyzon marinus),Rainbow trout (Salmo gairdnerii) and Bluegill (Lepomis macrochius) were used for the study.The study was performed under static conditions for 24 hrs. The test chemical conc. used for the study was 5 mg/l.

 Larval lampreys were collected by means of an electric shocker in the Ocqueoc River, Presque Isle County, Michigan, and were held in running water in aquaria and small “races” under conditions which simulated their natural stream habitat.

  Test fishes (Rainbow trout and Bluegill) were obtained fromthe stocks of local state and Federal fish hatcheriesand were held in large raceways. These specimens were maintained in the best possible physical condition until used in the laboratory.


The aggregate test animals available, usually six in number, were placed together in a 10-literglass battery jar containing 5 liters of water. The jars were provided with aeration through standard stone air-breakersand were maintained at a constant temperature by immersion in specially constructed constant temperature troughs. Water temperature was maintained within the limits of ± 1.0 °F.


Twelve of these test jars (each containing a substance being assayed) were included with one control jar in each trough. Fish and larvae in the control jar were exposed only to the water and physical conditions of the typical test container.

Test condition contains dissolved oxygen from 8.6 to 13.7 ppm and free CO2from 5.0 to 9.0 ppm

Observations of each test were measured approx. six times, at various intervals, during the 24 hr test period. At each observation, the condition of every test specimen was determined and recorded. Chronological histories were thus obtained of any symptoms of illness and the occurrence of death.

 No effects were observed when the test organisms sea lamprey, bluegill and rainbow trout were exposed to the test chemical for 24 hrs. Thus, the NOEC value was found to be 5 mg/l.


In other experimental data for same read across Data bank of Environmental Properties of Chemicals (EnviChem);2014 perform short term toxicity to fish the 48 hrs LC50 value to test fishes was found to be 335 mg/l.This LC50 value indicate that the test substance 2-Amino-8-naphthol-6-sulfonic acid is non toxic to fish as per the CLP criteria.(i.e value >100 mg/l)



Thus, overall weight of evidence studies from prediction model for target chemical (Cas no.130-23-4) and peer reviewed journals of read across substances (Cas no.87-02-5 and 90-51-7) indicate that the chemical 7-Amino-1-hydroxy-3-naphthalenesulfonic acid is non toxic to fish as per CLP criteria (i.e LC50 >100mg/l)and thus not consider for further classification.