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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Administrative data

Link to relevant study record(s)

Description of key information

A short-term toxicity test to aquatic invertebrates with the test substance can be waived because the requirements of Column 2 of Annex VII, Section 9.1.1, second indent are met, i.e. a long-term toxicity test with aquatic invertebrates with the test item is available in the registration dossier which provides an adequate and reliable coverage of the key parameter(s) of the corresponding OECD TG 211.

In addition, two reliable 48h EC50 values for invertebrates have been derived from related EDTA complexes, from which one (Borrmann, 2007) is based on measured data. This study is therefore selected as main supporting study. As the test from Borrmann resulted in an 48h EC50 of 100.9 mg EDTA-FeNa/L this result should be corrected for the Molecular Weight  (MW) difference in case the effects need to be compared. The MW ratio of EDTA-ZnK2/EDTA-FeNa is 1.176 (431.8/367.1) is used for the recalculation resulting in a 48h EC50 of 118.6 mg EDTA-ZnK2/L. 

Finally, the study from Sorvari and Sallinpaa confirm that EDTA-ZnK2 has a low acute ecotoxicity to daphnia 24h EC50 > 100 mg/L.

In this study the acute toxicity of EDTA, Mn(II)-EDTA, Fe(III)-EDTA, Cu(II)-EDTA, Zn(II)-EDTA, Hg(II)-EDTA and Cd(II)-EDTA  towards Daphnia magna was investigated according to ISO standard 6341. The EDTA-metal ion complexes were prepared by adding equimolar amounts of EDTA-Na4 and metal ion solutions together.

The 24h-EC50 values observed are:  17 mg/L expressed as Fe(III)  which equals 112 mg/L Fe(III)Na-EDTA, 38 mg/L expressed as Cu(II) which equals 259.5 mg/L CuK2-EDTA, 910 mg/L expressed as Zn(II) which equals 6010 mg/L Zn(II)K2-EDTA etc.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
118.2 mg/L

Additional information

Although multiple studies are available for daphnids, only two 48h studies are available, the other studies report either 24h or 96h results. Even a value for EDTA-Zn is available confirming the low toxicity to daphnids (24h EC50 = 1140 mg/L).

For this endpoint the 48h values are most relevant and both these values are in the same order of magnitude. As expected 24h EC50 values are somewhat higher, 96h EC50's show to be lower.