Registration Dossier
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EC number: 201-346-7 | CAS number: 81-39-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Adsorption / desorption
Administrative data
Link to relevant study record(s)
- Endpoint:
- adsorption / desorption: screening
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.19 (Estimation of the Adsorption Coefficient (KOC) on Soil and Sewage Sludge Using High Performance Liquid Chromatography (HPLC))
- Version / remarks:
- (adopted, May 2008)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC))
- Version / remarks:
- (adopted, January 2001)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Type of method:
- HPLC estimation method
- Media:
- soil/sewage sludge
- Radiolabelling:
- no
- Test temperature:
- The reference Koc-values in the OECD-guideline presumably refer to 25 °C. Even though the column temperature was 40 °C, this temperature is without influence on the result of the determination. The calibration was done referring to Koc- values of reference substances determined at 25 °C.
- Details on study design: HPLC method:
- I. Preparation of the test solutions:
- The dead time marker sodium nitrate was dissolved in acetonitrile / demineralized water 9:1 and diluted with acetonitrile
- The calibration substances were dissolved and diluted with acetonitrile
- The test item was dissolved in 5 ml tetrahydrofuran and diluted with acetonitrile
- The concentration for the calibration substances was approximately 0.03 to 0.1 mg/mL; the concentration for the test item was approximately 0.19 mg/mL
II. Equipment and chromatographic conditions
- Chromatograph: High Performance Liquid Chromatograph, HP 1100
- Column type: length: 250 mm, inner diameter: 4 mm
- Stationary Phase: LiChrospher 100 CN, particle diameter: 5 μm
- Supplier: Fa. Merck
- Mobile Phase: A: 830 mL demineralized water (Millipore) + 170 mL buffer pH 6 (Fluka, Art. No. 33545); B: Acetonitrile
- Flow rate: 1.0 mL/min
- Column temperature: 40 °C
- Detection: UV, 220 nm
- Injection volume: 3 μL
III pH determination (aqueous part of the mobile phase):
- Method no.: determined using a calibrated pH-meter with a single-rod glass electrode
- Result: 6.4
IV: Calibration substances:
- Sodium nitrate
- 2-Nitrobenzamide
- 3,5-Dinitrobenzamide
- Triazoxide
- 1,2,3-Trichlorobenzene
- Phenanthrene
- 9,10-Dimethyl-1,2-benz-anthracene - Key result
- Sample No.:
- #1
- Type:
- log Koc
- Value:
- 4.29
- pH:
- 6.4
- Temp.:
- 25 °C
- Remarks on result:
- other: Retention time [tr]: 8.8 minutes
- Remarks:
- Mean value of 3 single values
- Type:
- log Koc
- Value:
- 4.2
- Remarks on result:
- other: calculated
- Remarks:
- KOCWIN v. 2.00
- Validity criteria fulfilled:
- yes
- Conclusions:
- The adsorption coefficient of Solvent Red 52 was log Koc 4.29 measured at 25 °C and pH 6.4.
- Executive summary:
The adsorption coefficient (Koc) of Solvent Red 52 was determined according to the HPLC method at 40 °C. In order to comply with the guideline OECD 121 and considering environmental relevance, pH 6 was chosen as pH value for the buffer. The reference Koc-values in the OECD-guideline presumably refer to 25 °C. Even though the column temperature was 40 °C, this temperature is without influence on the result of the determination. The calibration was done referring to Koc- values of reference substances determined at 25 °C. For the determination of the adsorption coefficient (Koc) five calibration standards with log Koc from 1.45 - 5.37 are established in the EG-test method. The chromatogram of Macrolex Rot 5B showed the main signal eluting at 8.8 minutes. Because the non-ionic form of Solvent Red 52 exists at a pH value > 1.5 the test was carried out at approx. pH 6. The adsorption coefficient (log Koc) of Solvent Red 52 was 4.29 measured at 25 °C and pH 6.4.
Reference
Description of key information
The adsorption coefficient was determined by HPLC and the chromatogram showed
1 signal eluting at 8.8 minutes. The log Koc was calculated to be 4.29.
Key value for chemical safety assessment
- Koc at 20 °C:
- 19 498
Additional information
"Should read: log Koc 4.29 at 25 °C"
The reference Koc-values in the OECD-guideline presumably refer to 25 °C. Even though the column temperature was 40 °C, this temperature is without influence on the result of the determination. The calibration was done referring to Koc-values of reference substances determined at 25 °C.
[LogKoc: 4.29]
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