Dialuminium tris[2-(2,4,5,7-tetrabromo-6-oxido-3-oxoxanthen-9-yl)benzoate]

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

05.10.2016 to 07.10.2016

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

comparable to guideline study

Justification for type of information:

Data is from experimental reports following standard testing procedures.

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Principles of method if other than guideline:

Determination of the inhibition of the mobility of daphnids using OECD guideline 202.

GLP compliance:

not specified

Specific details on test material used for the study:

- Name of test material :- Dialuminium tris[2-(2,4,5,7-tetrabromo-6-oxido-3-oxoxanthen-9-yl)benzoate]
- Molecular formula :C20H8Br4O5.2/3Al
- Molecular weight :1991.5992 g/mol
- InChl :1S/3C20H8Br4O5.2Al/c3*21-11-5-9-17(13(23)15(11)25)28-18-10(6-12(22)16(26)14(18)24)20(9)8-4-2-1-3-7(8)19(27)29-20;;/h3*1-6,25-26H;;/q;;;2*+3/p-6
- Substance type: Organic
- Physical state:Solid

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: water flea
- Source:University of Chemistry and Technology Prague
- Feeding during test: Without feeding

Test type:

static

Water media type:

freshwater

Total exposure duration:

48 h

Test temperature:

20°C±1 °C

pH:

sample at concentration 200 mg/l:pH= 7.5 changed to pH=7.7during the test,
control 1: pH=7.6 changed to pH=7.4 during the test,
control 2: pH=7.7 didnot change during the test,
control 3 : pH=7.7 changed to pH=7.6 during the test

Dissolved oxygen:

higher than 7.9 mg/1 both in the control and the sample

Nominal and measured concentrations:

Nominal :10,25,50,100,200 mg/l

Details on test conditions:

TEST SYSTEM
- No. of organisms per vessel:5pieces
- No. of vessels per concentration (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water:The stock solution 200.0 mg/l was prepared by dissolving orange powder inreconstituted water.The stock solution was kept 5 min in ultrasonic bath.The test solutions of required concentrations were prepared by mixing the stock solution of the test sample inreconstituted test water.


OTHER TEST CONDITIONS
- Adjustment of pH:sample at concentration 200 mg/l:pH= 7.5 changed to pH=7.7during the test,
control 1: pH=7.6 changed to pH=7.4 during the test,
control 2: pH=7.7 didnot change during the test,
control 3 : pH=7.7 changed to pH=7.6 during the test
- Light intensity:darkness

TEST CONCENTRATIONS
- Test concentrations: 10,25,50,100,200 mg/l

Reference substance (positive control):

yes

Remarks:

K2Cr207

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

177.7 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: conf. level. (107.4-294.1) mg/l

Results with reference substance (positive control):

- Results with reference substance valid?
- Mortality: yes
- EC50: 0.79 mg/l
- Other:

Reported statistics and error estimates:

EC50 was calculated using nonlinear regression by the software Prism4.0 (GraphPadSoftware, Inc.,SanDiego CA)

Sample Name	Sample no.	Sample Information		c [mg/l]	I [%]	48 h EC50
Control 1,2,3	-	Reconstituted water		0	0	[mg/l]	95% CI [mg/l]
15	12912	CAS No. EC No.	15876-39-8 240-005-7	10.0 25.0 50.0 100.0 200.0	0 5.0 25.0 28.0 55.0	177.7	107.4–294.1

Conclusions:

The EC50 value for short term toxicity to Daphnia magna using OECD guideline 202 is 177.7 mg/l. It can be concluded from the value that the Dialuminium tris[2-(2,4,5,7-tetrabromo-6-oxido-3-oxoxanthen-9-yl)benzoate] is not toxic to the aquatic environment.

Executive summary:

Short term toxicity to aquatic invertebrates was performed in Daphnia magna following the OECD guideline 202. The test was performed for 48 hrs at different concentrations as control(1,2,3), 10, 25, 50, 100,200 mg/l .Reference substance used is K2Cr207.

5 pieces of daphnids after breeding were used in 4 numbers of replicates of chemical.The test was perfromed at a temperature of 20°C±1°C with taking 25ml per replicate in 50ml glass vessel for 48 hrs. Calulations of EC50 was calculated using nonlinear regression by the software Prism4.0 (GraphPadSoftware, Inc., SanDiegoCA).

The EC50 value for short term toxicity to Daphnia magna using OECD guideline 202 is 177.7 mg/l. It can be concluded from the value that the Dialuminium tris[2-(2,4,5,7-tetrabromo-6-oxido-3-oxoxanthen-9-yl)benzoate] is not toxic to the aquatic environment and can be considered as "not classified" as per CLP regulation.

Description of key information

The EC50 value for short term toxicity to Daphnia magna using OECD guideline 202 is 177.7 mg/l. It can be concluded from the value that the Dialuminium tris[2-(2,4,5,7-tetrabromo-6-oxido-3-oxoxanthen-9-yl)benzoate] is not toxic to the aquatic environment.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

177.7 mg/L

Additional information

Following study from experimental reports following standard testing procedures by ABITEC 2016, to conclude the toxicity extent of

Di aluminium tris [2-(2,4,5,7-tetrabromo-6-oxido-3-oxoxanthen-9-yl)benzoate] (CAS: 15876-39-8) towards aquatic invertebrate is summarized as follows:

Short term toxicity to aquatic invertebrates was performed in Daphnia magna following the OECD guideline 202. The test was performed for 48 hrs at different concentrations as control(1,2,3), 10, 25, 50, 100,200 mg/l .Reference substance used is K2Cr207.

5 pieces of daphnids after breeding were used in 4 numbers of replicates of chemical.The test was perfromed at a temperature of 20°C±1°C with taking 25ml per replicate in 50ml glass vessel for 48 hrs. Calulations of EC50 was calculated using nonlinear regression by the software Prism4.0 (GraphPadSoftware,Inc.,SanDiegoCA).

The EC50 value for short term toxicity to Daphnia magna using OECD guideline 202 is 177.7 mg/l. It can be concluded from the value that the Dialuminium tris[2-(2,4,5,7-tetrabromo-6-oxido-3-oxoxanthen-9-yl)benzoate] is not toxic to the aquatic environment and can be considered as "not classified" as per CLP regulation.