3,5,5-trimethylcyclohex-3-en-1-one

Toxicity to microorganisms

Administrative data

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: see 'Remark'

Remarks:

Study well documented, meets generally accepted scientific principles, acceptable for assessment. Data were taken from the structural analogue beta-Isophoron, CAS 78-59-1. The structural analogue differs in the location of the carbon double bound within the ring structure of the molecule, whereas the rest of the molecule is identical to CAS 471-01-2.

Data source

Reference

Reference Type:

publication

Title:

Evaluation of the test method -Activated sludge, respiration inhibition test proposed by the OECD

Author:

Yoshioka Y, Nagase H, Ose Y, Sato T

Year:

1986

Bibliographic source:

Ecotoxicol. Environ. Safety 12, 206-212

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

3,5,5-trimethylcyclohex-2-enone

EC Number:

201-126-0

EC Name:

3,5,5-trimethylcyclohex-2-enone

Cas Number:

78-59-1

IUPAC Name:

3,5,5-trimethylcyclohex-2-en-1-one

Sampling and analysis

Analytical monitoring:

not specified

Test organisms

Test organisms (species):

activated sludge of a predominantly domestic sewage

Details on inoculum:

TEST ORGANISMS
- Origin: Gifu-Hokubu Sewage Treatment Plant
- Pretreatment: washing with dist. water >= 3 times, suspended in dist. water at 4 g/l

Study design

Test type:

static

Water media type:

freshwater

Test conditions

Test temperature:

20 °C

Reference substance (positive control):

yes

Remarks:

3,5-dichlorophenol

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

3 h

Dose descriptor:

other: EC20

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Effect concentrations 2

Duration:

3 h

Dose descriptor:

EC50

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate