(E)-3-formylbut-2-enyl acetate

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Guideline study, comparable to OECD 203. Scientifically acceptable.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1989

Report date:

1989

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: German standard DIN 38 412 L15

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

(E)-3-formylbut-2-enyl acetate

EC Number:

247-825-4

EC Name:

(E)-3-formylbut-2-enyl acetate

Cas Number:

26586-02-7

Molecular formula:

C7H10O3

IUPAC Name:

(2E)-3-methyl-4-oxobut-2-en-1-yl acetate

Details on test material:

- Name of test material (as cited in study report): C5-acetat
- Lot/batch No.: B 838
- Analytical purity: about 99 %;
water solubility: slightly soluble according to sponsor.

Sampling and analysis

Analytical monitoring:

no

Test solutions

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A concentrated stock solution of the test substance in acetone (1.0 g/100 ml) was prepared and then added to the test aquaria. Then, the fish were placed into the test aquaria.

Test organisms

Test organisms (species):

Leuciscus idus

Details on test organisms:

TEST ORGANISM
- Common name: Golden orfe
- Source: Fischzucht Paul Eggers, 2354 Hohenweststedt, Germany
- Length at study initiation (length definition, mean, range and SD): 6.0 cm (range 5.5 - 7.1 cm)
- Weight at study initiation (mean and range, SD): 1.8 g (range 1.2 - 2.8 g)
- Corpulence factor of the test fish batch: 0.8
- Housing and adaption Culture conditions: oil-free aerated and charcoal filtered tap water, flow-through system; Water temperature: 20 - 21 °C Duration of housing: about 2 weeks Mortality during the time of housing: 0.4 %, Medical treatment: twice with 0.05 mg/l malachite green chloride, once with 10 mg/l tetracycline hydrochloride

ACCLIMATION
- Acclimation period: 3 days
- Type and amount of food: Growing feed F/B 50, ad libitum.
- Health during acclimation (any mortality observed): no mortality observed

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Hardness:

2.5 mmol/l

Test temperature:

21 - 23 °C

pH:

7.2 - 7.8

Dissolved oxygen:

6.8 - 7.8 mg/l

Nominal and measured concentrations:

Nominal test concentrations: 2.15, 4.64, 10.0 and 21.50 mg/l (based on the results of a range-finding test). In addition, a control was tested in parallel. No solvent control was tested in parallel, however, previous studies indicated that no effects occurred in solvent controls with 2.2 ml acetone/l test water.

Details on test conditions:

TEST SYSTEM
- Test vessel: All glass aquaria (30 x 22 x 24 cm)
- Fill volume: 10 l
- Aeration: slight
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 1.8 g fish/l test water


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted freshwater according to DIN 38412, Part II, 1982; prepared from fully demineralised tap water (conductivity: max 10 micro MHO) by adding: 294.0 mg/L CaCl2 * 2 H2O, 123.3 mg/L MgSO4 + 7 H2O, 63.0 mg/L NaHCO3, 5.5 mg/L KCl
- Ratio Na/K ions: 10 : 1
- Acid capacity: 0.8 mmol/l
- Ca/mg ratio: 4 : 1


OTHER TEST CONDITIONS
- Photoperiod: 16 : 8 hours day-night regime


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Mortality and symptoms of intoxication after 1, 4, 24, 48, 72 and 96 h.

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

10 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

10 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 3

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

14.7 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 4

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

21.5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Results with reference substance (positive control):

48 h-LC50 of the positive control Chloroacetamide: about 23 mg/l (corresponds to normal sensitivity).

Reported statistics and error estimates:

The EC50 value was calculated as the geometrical mean of 10.0 mg/l (LC0) and 21.50 mg/l (LC100) value.

Any other information on results incl. tables

Sublethal observations / clinical signs:

During the 96 h exposure to C5-acetat, mortalities were as follows:

Nominal test Cumulative mortalitiy (%) after x hours
concentration 1 / 4 / 24 / 48 / 72 / 96
(mg/l)
Control 0 / 0 / 0 / 0 / 0 / 0
2.150 0 / 0 / 0 / 0 / 0 / 0
4.640 5 / 0 / 0 / 0 / 0 / 0
10.000 10 / 0 / 0 / 0 / 0 / 0
21.50 0 / 0 / 10 / 10 / 10 / 10
Symptoms of intoxication:

Nominal test Symptoms of intoxication after x hours
concentration 1 / 4 / 24 / 48 / 72 / 96
(mg/l)
Control - / - / - / - / - / -
2.150 - / - / - / - / - / -
4.640 - / - / - / - / - / -
10.00 - / - / - / - / - / -
21.50 - / LT / + / + / + / +

Definitions of Symptoms:
-: No symptoms
+: All fish dead
L: Gasping
T: Tumbling