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Toxicological information

Basic toxicokinetics

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Administrative data

Endpoint:
basic toxicokinetics in vitro / ex vivo
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Test method was not according to any guideline. Monochloroacetic acid binding of in vitro sulfhydryl (SH) groups was examined as a possible mechanism of toxicity. No GLP.

Data source

Reference
Reference Type:
publication
Title:
Unnamed
Year:
1973

Materials and methods

Objective of study:
metabolism
Principles of method if other than guideline:
Monochloroacetic acid binding of in vitro sulfhydryl (SH) groups was examined as a possible mechanism of toxicity. In vitro binding of monochloroacetic acid to cysteine sulfhydryl groups was determined by a colorimetric sulfhydryl analysis (Sedlak and Lindsay, 1968). Test substance (60 µM) was added to an aqueous solution of 60 µM cysteine-HCl.
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): monochloroacetic acid

Test animals

Species:
rat
Strain:
Sprague-Dawley
Sex:
male
Details on test animals or test system and environmental conditions:
Rats were decapitated and their livers perfused via the central portal vein using 10 mL of 0.1 M Tris buffer (pH 7.4). Livers were excised, weighed and homogenized in 2 volumes 0.1 M Tris buffer (pH 7.4) using a motor-driven Potter-Elvehjem homogenizer.

Administration / exposure

Route of administration:
other: In vitro method
Vehicle:
water
Details on exposure:
Monochloroacetic acid was added to an aqueous solution of 60 µM cysteine-HCl.
Duration and frequency of treatment / exposure:
No data
Doses / concentrations
Remarks:
Doses / Concentrations:
60 and 120 µM
No. of animals per sex per dose / concentration:
In vitro method.
Control animals:
yes
Positive control reference chemical:
No data.

Results and discussion

Metabolite characterisation studies

Metabolites identified:
not measured

Any other information on results incl. tables

Monochloroacetic acid did not reduce significantly alkylate sulfhydryl groups of cysteine in vitro.

Applicant's summary and conclusion

Conclusions:
Interpretation of results (migrated information): other: MCA did not reduce significantly alkylate sulfhydryl groups of cysteine in vitro.
Monochloroacetic acid did not reduce significantly alkylate sulfhydryl groups of cysteine in vitro.
Executive summary:

Monochloroacetic acid binding of in vitro sulfhydryl (SH) groups was examined as a possible mechanism of toxicity.

In vitro binding of monochloroacetic acid to cysteine sulfhydryl groups was determined by a colorimetric sulfhydryl analysis (Sedlak and Lindsay, 1968). Test substance (60 µM) was added to an aqueous solution of 60 µM cysteine-HCl.

Monochloroacetic acid did not reduce significantly alkylate sulfhydryl groups of cysteine in vitro.