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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Basic toxicokinetics

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Administrative data

Endpoint:
basic toxicokinetics in vitro / ex vivo
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Test method was not according to any guideline. Monochloroacetic acid binding of in vitro sulfhydryl (SH) groups was examined as a possible mechanism of toxicity. No GLP.

Data source

Reference
Reference Type:
publication
Title:
Unnamed
Year:
1973

Materials and methods

Objective of study:
metabolism
Principles of method if other than guideline:
Monochloroacetic acid binding of in vitro sulfhydryl (SH) groups was examined as a possible mechanism of toxicity. In vitro binding of monochloroacetic acid to cysteine sulfhydryl groups was determined by a colorimetric sulfhydryl analysis (Sedlak and Lindsay, 1968). Test substance (60 µM) was added to an aqueous solution of 60 µM cysteine-HCl.
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): monochloroacetic acid

Test animals

Species:
rat
Strain:
Sprague-Dawley
Sex:
male
Details on test animals or test system and environmental conditions:
Rats were decapitated and their livers perfused via the central portal vein using 10 mL of 0.1 M Tris buffer (pH 7.4). Livers were excised, weighed and homogenized in 2 volumes 0.1 M Tris buffer (pH 7.4) using a motor-driven Potter-Elvehjem homogenizer.

Administration / exposure

Route of administration:
other: In vitro method
Vehicle:
water
Details on exposure:
Monochloroacetic acid was added to an aqueous solution of 60 µM cysteine-HCl.
Duration and frequency of treatment / exposure:
No data
Doses / concentrations
Remarks:
Doses / Concentrations:
60 and 120 µM
No. of animals per sex per dose / concentration:
In vitro method.
Control animals:
yes
Positive control reference chemical:
No data.

Results and discussion

Metabolite characterisation studies

Metabolites identified:
not measured

Any other information on results incl. tables

Monochloroacetic acid did not reduce significantly alkylate sulfhydryl groups of cysteine in vitro.

Applicant's summary and conclusion

Conclusions:
Interpretation of results (migrated information): other: MCA did not reduce significantly alkylate sulfhydryl groups of cysteine in vitro.
Monochloroacetic acid did not reduce significantly alkylate sulfhydryl groups of cysteine in vitro.
Executive summary:

Monochloroacetic acid binding of in vitro sulfhydryl (SH) groups was examined as a possible mechanism of toxicity.

In vitro binding of monochloroacetic acid to cysteine sulfhydryl groups was determined by a colorimetric sulfhydryl analysis (Sedlak and Lindsay, 1968). Test substance (60 µM) was added to an aqueous solution of 60 µM cysteine-HCl.

Monochloroacetic acid did not reduce significantly alkylate sulfhydryl groups of cysteine in vitro.