3-methoxyacetophenone

Endpoint summary

Description of key information

Short term toxicity to fish:

After 96 hours of exposure to test item ( 3-methoxyacetophenone ) to nominal test concentration, LC50 was determine to be 100 mg/l . Based on the LC50, it can be consider that the chemical was not hazardous and can be consider to be not classified as per the CLP classification criteria.

 

Short term toxicity to aquatic invertebrates:

Aim of this study was to assess the short term toxicity of m-Methoxyacetophenone to aquatic invertebrate daphnia magna. Study was performed according to the OECD guideline in a static system for the total exposure period of 48 hrs. The stock solution 100 mg/l was prepared by dissolving transparent liquid in reconstituted water. Test solutions of required concentration as were prepared by mixing the stock solution of the test sample with reconstituted test water. 0, 3, 6, 12, 24, 50 and 100 mg/l concentrations were prepared and used in the study. Effects on immobilisation were observed for 48 hours. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0. The median effective concentration (EC50) for the test substance, m-Methoxyacetophenone, in Daphnia magna was determined to be 21.4 mg/L on the basis of mobility inhibition effects in a 48 hour study. Thus the EC50 value, indicates that the substance is likely to be hazardous to aquatic invertebrates and can be classified as aquatic chronic 3 category as per the CLP criteria. But as the chemical readily degrade when comes in contact with water thus on that basis it was consider as nontoxic and not classified as per the CLP classification criteria.

Toxicity to aquatic algae:

Freshwater algal growth inhibition test was carried out on Desmodesmus subspicatus with the substance 3-methoxyacetophenone according to OECD Guideline 201. The stock solution 200 mg/L was prepared by dissolving transparent liquid in OECD growth medium. Test solutions of required concentrations were prepared by mixing the stock solution of the test sample with OECD growth medium and inoculum culture and tested at the concentrations 0, 2.2, 11, 25, 55 and 120 mg/L. Effects on the growth rate of the organism were studied. Study consider as valid as it fulfills all necessary criteria and follows OECD guideline. The median effective concentration (ErC50) for the test substance, 3-methoxyacetophenone, on Desmodesmus subspicatus was determined to be 129.3 mg/L. Based on this ErC50 value and after comparing with CLP criteria for aquatic classification of the substance it is concluded that the substance 3-methoxyaceto phenone does not exhibit toxicity to aquatic algae (Desmodesmus subspicatus) and not classified as per the CLP classification criteria.

Toxicity to microorganisms:

The Minimum Inhibitory Concentration (MIC) of Bacillus subtilis NCIM 2718 and Salmonella typhi NCIM2501 for 3-Methoxyacetophenone (586-37-8) was determined as 0.0629 mg/l.

Additional information

Short term toxicity to fish:

Study was conducted to access the effect of test chemical 3-methoxyacetophenone on the growth of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test). The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 400 mg of the test substance in 4 liters of potable water (passed through reverse osmosis system) with continuous stirring. This test solution was then added to the remaining three liters of water for achieving test concentrations of 100 mg/L and Zebra FishDanio reriowere exposed to these concentration for 96 hours. Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 96 hours of exposure to test item ( 3-methoxyacetophenone ) to nominal test concentration, LC50 was determine to be 100 mg/l . Based on the LC50, it can be consider that the chemical was not hazardous and can be consider to be not classified as per the CLP classification criteria.

 

Short term toxicity to aquatic invertebrates:

Aim of this study was to assess the short term toxicity of m-Methoxyacetophenone to aquatic invertebrate daphnia magna. Study was performed according to the OECD guideline in a static system for the total exposure period of 48 hrs. The stock solution 100 mg/l was prepared by dissolving transparent liquid in reconstituted water. Test solutions of required concentration as were prepared by mixing the stock solution of the test sample with reconstituted test water. 0, 3, 6, 12, 24, 50 and 100 mg/l concentrations were prepared and used in the study. Effects on immobilisation were observed for 48 hours. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0. The median effective concentration (EC50) for the test substance, m-Methoxyacetophenone, in Daphnia magna was determined to be 21.4 mg/L on the basis of mobility inhibition effects in a 48 hour study. Thus the EC50 value, indicates that the substance is likely to be hazardous to aquatic invertebrates and can be classified as aquatic chronic 3 category as per the CLP criteria. But as the chemical readily degrade when comes in contact with water thus on that basis it was consider as nontoxic and not classified as per the CLP classification criteria.

Toxicity to aquatic algae:

Freshwater algal growth inhibition test was carried out on Desmodesmus subspicatus with the substance 3-methoxyacetophenone according to OECD Guideline 201.

The stock solution 200 mg/L was prepared by dissolving transparent liquid in OECD growth medium. Test solutions of required concentrations were prepared by mixing the stock solution of the test sample with OECD growth medium and inoculum culture and tested at the concentrations 0, 2.2, 11, 25, 55 and 120 mg/L. Effects on the growth rate of the organism were studied. Study consider as valid as it fulfills all necessary criteria and follows OECD guideline. The median effective concentration (ErC50) for the test substance, 3-methoxyacetophenone, on Desmodesmus subspicatus was determined to be 129.3 mg/L. Based on this ErC50 value and after comparing with CLP criteria for aquatic classification of the substance it is concluded that the substance 3-methoxyaceto phenone does not exhibit toxicity to aquatic algae (Desmodesmus subspicatus) and not classified as per the CLP classification criteria.

Toxicity to microorganisms:

From peer reviwed journal (Chem Biol Drug Des 2008; 72: 303–313) the Antibacterial activity of 3-Methoxyacetophenone was observed for microorganism determined by minimum inhibitory concentration using by two-fold dilution method.

 

Hydrophobicity was observed for the bacteria more hydrophobic the bacterial cell, the greater is its affinity to the hydrocarbon, resulting in a transfer of cells from the aqueous suspension to the organic phase, leading to a reduction in the turbidity of the culture in the former.

Optical density (OD) was measured at concentrations from 0.0 to 0.2ml.

Bacteria were cultured in medium at mid-logarithmic phase and followed the protocol. OD was measured at 400 nm and cell-free buffer served as the blank.A plot was made between OD and different hexadecane concentrations.

 

After experiment it was determined thatthe Minimum Inhibitory Concentration (MIC) of Bacillus subtilis NCIM 2718 and Salmonella typhi NCIM2501 for3-Methoxyacetophenone (586-37-8)was determined as 0.0629 mg/l.

 

Other supporting study from same experimental result for different species i.e Staphylococcus aureus NCIM5021, Enterobacte aerogenes NCIM5139 and Proteus vulgaris NCIM2813 showed Minimum Inhibitory Concentration (MIC) as 0.0249 mg/l and 0.0011 mg/l respectively.

 

Overall experimental studies from peer reviwed articles indicate that the test substance 3-Methoxyacetophenone (586-37-8) showed minimum inhibitory effects in various micro-organisms in range 0.0011 - 0.0629 mg/l.