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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Justification for type of information:
Guideline study to GLP certification with no deviations.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2017
Report date:
2017

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
5-chloropentan-2-one
EC Number:
227-565-8
EC Name:
5-chloropentan-2-one
Cas Number:
5891-21-4
Molecular formula:
C5H9ClO
IUPAC Name:
5-chloropentan-2-one
Test material form:
liquid
Remarks:
dark brown
Specific details on test material used for the study:
SOURCE OF TEST MATERIAL
- Source and lot/batch number of test material:
5-Chloropentan-2-one (CAS-No.: 5891-21-4), Sanofi Chimie
- Purity: 95.9%

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
- Concentrations:
Nominal concentrations were 100, 45.5, 20.7, 9.4 and 4.3 mg/L
Geometric mean concentrations were 81.8, 39.8, 18.8, 8.43 and 3.06 mg/L

Samples collected from all test concentrations for analysis at 0, 24 and 48 hours.

Test solutions

Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
The test media were prepared just before introduction of the daphnids (= start of the test) and test medium renewal on Day 1.

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name:
Water flea
- Strain/clone:
Daphnia magna (Straus), clone 5
- Age at study initiation (mean and range, SD):
From 3.5 to 21.0 hours old

Study design

Test type:
semi-static
Water media type:
freshwater
Remarks:
Elendt M4 medium
Limit test:
no
Total exposure duration:
48 h
Remarks on exposure duration:
None
Post exposure observation period:
None

Test conditions

Hardness:
No data (Elendt M4 medium)
Test temperature:
19.1 to 20.1 °C across the test concentrations, replicates and test duration.
pH:
7.5 to 8.1 across the test concentrations, replicates and test duration.
Dissolved oxygen:
8.3 to 9.1 mg/L across the test concentrations, replicates and test duration.
Salinity:
Not applicable
Conductivity:
No data (Elendt M4 medium)
Nominal and measured concentrations:
Nominal concentrations were 100, 45.5, 20.7, 9.4 and 4.3 mg/L
Geometric mean concentrations were 81.8, 39.8, 18.8, 8.43 and 3.06 mg/L
Details on test conditions:
TEST SYSTEM
- Renewal rate of test solution (frequency/flow rate):
Once at 24 hours
- No. of organisms per vessel:
5
- No. of vessels per concentration (replicates) 4:
- No. of vessels per control (replicates): 4

OTHER TEST CONDITIONS
- Photoperiod:
16 h light : 8 h dark
Reference substance (positive control):
yes
Remarks:
potassium dichromate

Results and discussion

Effect concentrationsopen allclose all
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 81.8 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC10
Effect conc.:
> 81.8 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
81.8 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
LOEC
Effect conc.:
> 81.8 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
All reported results refer to geometric mean measured concentrations, since the test item concentrations were not within ± 20% of the nominal or measured initial concentrations during the test.
Results with reference substance (positive control):
EC50 after 24 hours was determined to be 1.48 mg test item/L, indicating that the sensitivity of the daphnids was consistent with the level proposed by the OECD 202 guideline (EC50-24 h between 0.6 and 2.1 mg potassium dichromate/L).
Reported statistics and error estimates:
The 24-hour and 48-hour EC50, EC20 and EC10 and the 95 % confidence limits were calculated by probit analysis. The NOEC and LOEC after 24 and 48 hours were determined directly from the raw data.
The software used to perform the statistical analysis was ToxRat Professional, Version 3.2.1, ToxRat Solutions GmbH.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The reliable (K1) OECD 202 Guideline study found that acute toxicity was observed in Daphnia magna resulting in a 48-hr EC50 of >81.8 mg/L. based on measured geometric mean.
Executive summary:

The reliable (K1) OECD 202 Guideline study found that acute toxicity was observed in Daphnia magna resulting in a 48-hr EC50 of >81.8 mg/L. based on measured geometric mean. Other endpoint results are as follows:


48h-NOEC = 81.8 mg/L
48h-LOEC = >81.8 mg/L
48h-EC10= >81.8 mg/L