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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
17-05-2017 - 19-05-2017
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Justification for type of information:
Experimental test result performed using standard test guidelines
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
no
Analytical monitoring:
no
Vehicle:
no
Details on test solutions:
The stock solution (120 mg/L) was prepared by dissolving clear yellow coloured iquid in reconstituted water. Test solutions of required concentrationas were prepared by mixing the stock solution of the test sample with reconstituted test water.
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Water flea
- Strain: Straus
- Source: Own breeding at University of Chemistry and Technology, Prague
- Age at study initiation (mean and range, SD): The animals used for the test shall be less than 24 h old and should not be first brood progeny
- Feeding during test: No feeding

ACCLIMATION - No data available
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Remarks on exposure duration:
± 1 hr
Hardness:
not specified
Test temperature:
20±1°C
pH:
Without adjustment
Sample at concentration 120mg/L: pH= 7.6 changed to pH=8.2 during the test
Control: pH = 7.6 changed to pH=8.1 during the test
Dissolved oxygen:
higher than 8.8 mg/L at the end of test
Salinity:
not specified
Conductivity:
not specified
Nominal and measured concentrations:
3.8, 7.5, 15, 30, 60 and 120 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: 50 ml glass vessel
- fill volume: 25 ml
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water:
Natural water (surface or ground water), reconstituted water or dechlorinated tap water are acceptable as culturing and dilution water if D. magna survives in it for the duration of the culturing, acclimation and testing without showing signs of stress. Waters in the range pH 6 to pH 9, with hardness between 140 mg/l and 275 mg/l (as CaCO3) are recommended.
As an example, the preparation of dilution water meeting the requirements is described below.
Dissolve known quantities of reagents in water. The dilution water prepared shall have a pH of 7.8 ± 0.5, a hardness of (225 ± 50) mg/l (expressed as CaCO3), a molar Ca + Mg ratio close to 4 + 1 and a dissolved oxygen concentration above 7 mg/l.

Prepare the solutions specified below:
- Calcium chloride solution: Dissolve 117.6 g of calcium chloride dihydrate (CaCl2.2H2O) in water (4.2) and make up to 1 l with water (4.2).
- Magnesium sulfate solution: Dissolve 49.3 g of magnesium sulfate heptahydrate (MgSO4.7H2O) in water (4.2) and make up to 1 l with water (4.2).
- Sodium bicarbonate solution: Dissolve 25.9 g of sodium bicarbonate (NaHCO3) in water (4.2) and make up to 1 l with water (4.2).
- Potassium chloride solution: Dissolve 2.3 g of potassium chloride (KCI) in water (4.2) and make up to 1 l with water (4.2).

Mixing
Mix 2.5 ml of each of the four solutions and make up to 1 l with water.
The dilution water shall be aerated until the dissolved oxygen concentration has reached saturation and the pH has stabilized. If necessary, adjust the pH to 7.8 ± 0.5 by adding sodium hydroxide (NaOH) solution or hydrochloric acid (HCI). The dilution water prepared in this way shall not be further aerated before use.

- Sodium hydroxide solution, e.g. [NaOH] : 1 mol/l.
- Hydrochloric acid, e.g. [HCl] : 1 mol/l.

Reference substance:
Dissolve 600 mg of potassium dichromate (K2Cr2O7) in water and make up to 1 l with water (4.2).

OTHER TEST CONDITIONS
- Adjustment of pH: no adjustment done
- Photoperiod: No - Darkness
- Light intensity:

CALCULATION:
EC50 was calculated using non linear regression by the software Prism 4.0
Reference substance (positive control):
yes
Remarks:
Potassium dichromate (K2Cr2O7)
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
59.6 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: 95% CL: 43.4 - 81.8 mg/L
Results with reference substance (positive control):
- Results with reference substance valid
- EC50: 0.73 mg/L (24 hours)
Reported statistics and error estimates:
not specified
Validity criteria fulfilled:
yes
Conclusions:
The median effective concentration (EC50) for the test substance, in Daphnia magna was determined to be 59.6 mg/L on the basis of mobiity inhibition effects in a 48 hour study.
Executive summary:

Determination of the inhibition of the mobility of daphnids was carried out with the substance according to OECD Guideline 202.The test substance was tested at the concentrations 3.8, 7.5, 15, 30, 60 and 120 mg/L. Effects on immobilisation were observed for 48 hours. The stock solution (120 mg/L) was prepared by dissolving clear yellow coloured iquid in reconstituted water. Test solutions of required concentrationas were prepared by mixing the stock solution of the test sample with reconstituted test water.The median effective concentration (EC50) for the test substance, 2 -Octanone, in Daphnia magna was determined to be 59.6 mg/L for immobilisation effects.This value indicates that the test substance is likely to be hazardous to aquatic invertebrates , hence it can be classified as Aquatic Chronic category 3 as per the CLP criteria.

Description of key information

Short term toxicity to aquatic invertebrates:

Determination of the inhibition of the mobility of daphnids was carried out with the substance according to OECD Guideline 202.The test substance was tested at the concentrations3.8, 7.5, 15, 30, 60 and 120 mg/L. Effects on immobilisation were observed for 48 hours.The stock solution (120 mg/L) was prepared by dissolving clear yellow coloured iquid in reconstituted water. Test solutions of required concentrationas were prepared by mixing the stock solution of the test sample with reconstituted test water.The median effective concentration (EC50) for the test substance, 2 -Octanone, in Daphnia magna was determined to be 59.6 mg/L for immobilisation effects.This value indicates that the test substance is likely to be hazardous to aquatic invertebrates , hence it can be classified as Aquatic Chronic category 3 as per the CLP criteria.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
59.6 mg/L

Additional information

Short term toxicity to aquatic invertebrates:

Determination of the inhibition of the mobility of daphnids was carried out with the substance according to OECD Guideline 202.The test substance was tested at the concentrations3.8, 7.5, 15, 30, 60 and 120 mg/L. Effects on immobilisation were observed for 48 hours.The stock solution (120 mg/L) was prepared by dissolving clear yellow coloured iquid in reconstituted water. Test solutions of required concentrationas were prepared by mixing the stock solution of the test sample with reconstituted test water.The median effective concentration (EC50) for the test substance, 2 -Octanone, in Daphnia magna was determined to be 59.6 mg/L for immobilisation effects.This value indicates that the test substance is likely to be hazardous to aquatic invertebrates , hence it can be classified as Aquatic Chronic category 3 as per the CLP criteria.