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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Environmental fate & pathways

Biodegradation in water: screening tests

Administrative data

biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Guideline Study with detailed documentation

Data source

Reference Type:
study report
Report date:

Materials and methods

Test guidelineopen allclose all
according to guideline
OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
according to guideline
EU Method C.4-C (Determination of the "Ready" Biodegradability - Carbon Dioxide Evolution Test)
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Reference substance name:
Details on test material:
- Name of test material (as cited in study report): MAEEBP- Physical state: colourless liquid- Composition of test material, percentage of components: 4-methacryloyl diethoxy benzophenone 85 area%, impurities 4-hydroxybenzophenone <5%, 4-methacryloyl benzophenone <5%, polymethacrylate <5%- Lot/batch No.: 150301- Expiration date of the lot/batch: Sep. 2015- Storage condition of test material: The test item was stored in the test facility in a closed vessel in the refrigerator (3.9-9.2°C) (exception: 22.- 28. Apr. 2015 storage at room temperature).

Study design

Oxygen conditions:
Inoculum or test system:
activated sludge, domestic, non-adapted
Details on inoculum:
- Source of inoculum/activated sludge: Activated sludge from a biologic sewage treatment plant of the ESN (Stadtentsorgung Neustadt), Germany- Pre-treatment: The sludge was filtrated, washed with tap water twice, then washed with and re-suspended in test medium. It was then aerated until use.- Concentration of sludge: 4540 mg suspended solids/L (dry matter)
Duration of test (contact time):
29 d
Initial test substance concentration
Initial conc.:
20 mg/L
Based on:
Parameter followed for biodegradation estimation
Parameter followed for biodegradation estimation:
CO2 evolution
Details on study design:
TEST SYSTEM- Culturing apparatus: 2000 mL-SCHOTT-flasks were used as test vessels, 100 mL scrubber flasks as absorbent vessels. Flask volume 1500 mL- Number of culture flasks/concentration: 2- Method used to create aerobic conditions: The test vessels were filled with medium and inoculum. Then, all flasks were aerated for 72 hours with purified, CO2-free, moistened air to purge the system of CO2.- Measuring equipment: carbon analyser TOC multi N/C 2100S, Analytik JenaSAMPLING- Sampling frequency: on day 0, 2, 4, 7, 9, 11, 14, 18, 23 and 29CONTROL AND BLANK SYSTEM- Inoculum blank: 2 flasks- Reference: 2 flasks- Abiotic sterile control: 1 flask (HgCl2)- Toxicity control: 1 flask - Other: Apparatus blanks, 2 flasks, containing mineral medium only
Reference substance
Reference substance:

Results and discussion

% Degradation
% degradation (CO2 evolution)
Sampling time:
28 d

Any other information on results incl. tables

Degradation values after 29 d:

Positive control mean: 86.8%

Test mean: 42.7%

Abiotic control: -1.4%

Toxicity control: 53.7%

10-day-window: day 12 – 22

Degradation at the end of 10-day-window 25 %

Applicant's summary and conclusion

Validity criteria fulfilled:
Interpretation of results:
inherently biodegradable
The test item MAEEBP was tested using a concentration of nominally 20 mg organic carbon/L (corresponding to 28.5 mg MAEEBP/L) in test medium following OECD 301B and EU-Method C.4-C. The test item degraded to 43% within 28 days. Therefore, regardless of the 10-day-window, MAEEBP is not readily biodegradable following OECD 301B/EU C.4-C.