Methylammonium chloride

Toxicity to aquatic algae and cyanobacteria

Link to relevant study record(s)

Reference

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Data from peer review journal

Justification for type of information:

Data from peer review journal

Qualifier:

according to guideline

Guideline:

other: as per mentioned below

Principles of method if other than guideline:

Short term toxicity to Microcystis aeruginosa study was carried out for 24 hrs.

GLP compliance:

not specified

Specific details on test material used for the study:

- Name of test material (as cited in study report): Methylamine hydrochloride
- Molecular formula (if other than submission substance): CH5N.Cl H
- Molecular weight (if other than submission substance): 67.5184 g/mol
- Smiles notation (if other than submission substance): C[NH3+].[ClH-]
- InChl (if other than submission substance): 1S/CH5N.ClH/c1-2;/h2H2,1H3;1H
- Substance type: Organic
- Physical state: Solid

Analytical monitoring:

not specified

Details on sampling:

- Concentrations: 20 mg/l (nominal concentrations)
- Sampling method: Test chemical was prepared in stock solutions and aliquots added to individual cultures to give the desired concentrations.

Vehicle:

not specified

Test organisms (species):

Microcystis aeruginosa

Details on test organisms:

TEST ORGANISM
- Common name: Blue-Green Algae
- Age of inoculum (at test initiation): Exponential Growth Phase (Log)
- Method of cultivation: The algae was grown in 125 ml Erlenmeyer flasks with 75 ml of a modified Chu No. 10 solution under continuous florescent illumination and at a temperature of approx. 22ᵒC, respectively.

Test type:

static

Water media type:

freshwater

Total exposure duration:

24 h

Test temperature:

22°C

Nominal and measured concentrations:

nominal concentrations

Details on test conditions:

TEST SYSTEM
- Test vessel: Erlenmeyer flask
- Material, size, headspace, fill volume: Erlenmeyer flasks of 125 ml was used as a test vessel.
- Initial cells density: 1,000,000 to 2,000,000 cells/ml

GROWTH MEDIUM
- Detailed composition if non-standard medium was used: The composition of the nutrient solution in gm/l was as follows-
NaNO3, 0.124; K2HPO4.3H2O, 0.013; MgSO4.7H2O, 0.025; Na2CO3, 0.020; Na2SiO3.9H2O, 0.058; CaCl2.2H2O, 0.036; ferric citrate, 0.003; and citric acid, 0.003.


OTHER TEST CONDITIONS
- Light intensity and quality: continuous florescent illumination

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: Microscopic examination of the cells were carried out for determining the extent of their disintegration after 24 hr period.
- Other: The failure of treated cultures to grow in subcultures was used for determining the minimum lethal dosages.

TEST CONCENTRATIONS
- Test concentrations: 20 mg/l

Reference substance (positive control):

not specified

Duration:

24 h

Dose descriptor:

EC100

Effect conc.:

20 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Table: Effect of test chemical Methylamine hydrochloride on the growth of Microcystis aeruginosa.

 

Chemical	Conc. (mg/l)	Kill (%)
Methylamine.HCl	20	100

 

Validity criteria fulfilled:

not specified

Conclusions:

For chemical Methylamine hydrochloride, based on the effect on growth rate of the test organism Microcystis aeruginosa, the 24 hr EC100 value was determined to be 20 mg/l, respectively.

Executive summary:

Short term toxicity to Microcystis aeruginosa study was carried out for 24 hrs.The study was based on the effects of the test compound Methylamine hydrochloride (CAS no. 593 -51 -1) on Microcystis aeruginosa in a static fresh water system at a temperature of 22ᵒC. Microcystis aeruginosa was used as a test organism. The algae was grown in 125 ml Erlenmeyer flasks with 75 ml of a modified Chu No. 10 solution under continuous florescent illumination and at a temperature of approx. 22ᵒC, respectively. Test chemical conc. used for the study was 20 mg/l(nominal concentrations). Test chemical was prepared in stock solutions and aliquots added to individual cultures to give the desired concentrations. Test was performed in a 125 ml Erlenmeyer flasks under continuous florescent illumination at a temperature of 22ᵒC. Initial cell of the organism used for the study was 1,000,000 to 2,000,000 cells/ml, respectively. Microscopic examination of the cells were carried out for determining the extent of their disintegration after 24 hr period. The failure of treated cultures to grow in subcultures was used for determining the minimum lethal dosages. Based on the effect on growth rate of the test organism Microcystis aeruginosa, the 24 hr EC100 value was determined to be 20 mg/l, respectively. Thus, based on the EC100 value, it can be concluded that the substance Methylamine hydrochloride can be considered as toxic to aquatic organisms. Since, the chemical Methylamine hydrochloride is readily biodegradable in nature, Methylamine hydrochloride can be considered as non-toxic to aquatic organisms and thus cannot be classified as hazardous as per the CLP classification criteria.

Description of key information

Freshwater algal growth inhibition test was carried out on Desmodesmus subspicatus with the substance methanaminium chloride (CAS no. 593 -51 -1) according to OECD Guideline 201 (ABITEC study report, Sustainability Support Services (Europe) AB, Report no. 715/16, 2016) . The stock solution (300 mg/L) was prepared by dissolving white powder in OECD growth medium. Test solutions  of required concentrations  were prepared by mixing the stock solution of the test sample with OECD growth medium and inoculum culture. Conc. of test chemical used for the study was 0, 60, 90, 132, 201 and 300 mg/L, respectively. The test was performed under static conditions in a static fresh water system at a temp. of 23±2°C. Initial cell density of test organism used was 5x10(3) cells/ml. Determination of cell counting involve the use of electronic particle counter. ErC50 was calculated using non-linear regression by the software Prism 4.0. The median effective concentration (EC50) for the test substance methanaminium chloride in Desmodesmus subspicatus was determined to be 971.6 mg/L on the basis of effects on growth rate in a 72 hour study. Thus, based on this value, test chemical methanaminium chloride can be considered as non-toxic to aquatic organisms and thus can be considered to be not classified as per the CLP classification criteria.

Key value for chemical safety assessment

EC50 for freshwater algae:

971.6 mg/L

Additional information

1 experimental key study alongwith the supporting datafor the target chemical methanaminium chloride(CAS no. 593-51-1)were reviewed for the toxicity to aquatic algae and cyanobacteria end point which are summarized as below:

 

In an experimental key study (ABITEC study report, Sustainability Support Services (Europe) AB, Report no. 715/16, 2016), Freshwater algal growth inhibition test was carried out on Desmodesmus subspicatus with the substance methanaminium chloride (CAS no. 593 -51 -1) according to OECD Guideline 201. The stock solution (300 mg/L) was prepared by dissolving white powder in OECD growth medium. Test solutions of required concentrations were prepared by mixing the stock solution of the test sample with OECD growth medium and inoculum culture. Conc. of test chemical used for the study was 0, 60, 90, 132, 201 and 300 mg/L, respectively. The test was performed under static conditions in a static fresh water system at a temp. of 23±2°C. Initial cell density of test organism used was 5x10(3) cells/ml. Determination of cell counting involve the use of electronic particle counter. ErC50 was calculated using non-linear regression by the software Prism 4.0. The median effective concentration (EC50) for the test substance methanaminium chloride in Desmodesmus subspicatus was determined to be 971.6 mg/L on the basis of effects on growth rate in a 72 hour study. Thus, based on this value, test chemical methanaminium chloride can be considered as non-toxic to aquatic organisms and thus can be considered to be not classified as per the CLP classification criteria.

 

In a supporting data (Fitzgerald,G.P., G.C. Gerloff, and F. Skoog, 1952) of short term toxicity to Microcystis aeruginosa for the test compound Methylamine hydrochloride (CAS no. 593 -51 -1) was carried out for 24 hrs in a static fresh water system at a temperature of 22ᵒC. Microcystis aeruginosa was used as a test organism. The algae was grown in 125 ml Erlenmeyer flasks with 75 ml of a modified Chu No. 10 solution under continuous florescent illumination and at a temperature of approx. 22ᵒC, respectively. Test chemical conc. used for the study was 20 mg/l(nominal concentrations). Test chemical was prepared in stock solutions and aliquots added to individual cultures to give the desired concentrations. Test was performed in a 125 ml Erlenmeyer flasks under continuous florescent illumination at a temperature of 22ᵒC. Initial cell of the organism used for the study was 1,000,000 to 2,000,000 cells/ml, respectively. Microscopic examination of the cells were carried out for determining the extent of their disintegration after 24 hr period. The failure of treated cultures to grow in subcultures was used for determining the minimum lethal dosages. Based on the effect on growth rate of the test organism Microcystis aeruginosa, the 24 hr EC100 value was determined to be 20 mg/l, respectively. Thus, based on the EC100 value, it can be concluded that the substance Methylamine hydrochloride can be considered as toxic to aquatic organisms. Since, the chemical Methylamine hydrochloride is readily biodegradable in nature, Methylamine hydrochloride can be considered as non-toxic to aquatic organisms and thus cannot be classified as hazardous as per the CLP classification criteria.

 

Thus, based on the overall reported results for target chemicalmethanaminium chloride(from ABITEC study report and peer reviewed journal), it can be concluded that the test substance methanaminium chloride can be considered as non-toxic to aquatic environment and can be considered to be not classified as per the CLP classification criteria.